Serotonin-mediated modulation of hypoxia-induced intracellular calcium responses in glomus cells isolated from rat carotid body
Serotonin-mediated modulation of hypoxia-induced intracellular calcium responses in glomus cells isolated from rat carotid body
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DOI：
10.1016/j.neulet.2015.04.044
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发表时间：
2015-06
影响因子：
2.5
通讯作者：
Takuya Yokoyama;N. Nakamuta;T. Kusakabe;Yoshio Yamamoto
中科院分区：
文献类型：
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作者：
Takuya Yokoyama;N. Nakamuta;T. Kusakabe;Yoshio Yamamoto
在本研究中，我们检测了从大鼠颈动脉体 (CB) 中分离出的血管球细胞中血清素 (5-HT) 诱导的细胞内 Ca2+([Ca2+]i) 对缺氧的反应。在常氧（21% O2）期间，5-HT 不会诱导聚集的血管球细胞中任何 [Ca2+] 反应，而灌注低氧溶液（1% O2）会诱导同一样本中 [Ca2+]i 重复增加。在 5-HT 存在的情况下，血管球细胞中观察到的缺氧诱导的 [Ca2+] 变化的频率和幅度增强，并且这种反应被 5-HT2 受体拮抗剂酮色林抑制。此外，RT-PCR 分析检测了 CB 提取物中 5-HT1A、5-HT1B、5-HT1D、5-HT1F、5-HT2A、5-HT2B、5-HT3A 和 5-HT3B 受体 mRNA 的表达。这些结果表明 5-HT 增加血管球细胞中缺氧诱导的 [Ca2+] 反应。 5-HT 可能会增强血管球细胞的缺氧反应，从而增加 CB 的化学感应活性。
In the present study, we examined serotonin (5-HT)-induced intracellular Ca2+([Ca2+]i) responses to hypoxia in glomus cells isolated from carotid body (CB) of the rat. 5-HT did not induce any [Ca2+]iresponses in clustered glomus cells during normoxia (21% O2), whereas, the perfusion of hypoxic solution (1% O2) induced repetitive increases in [Ca2+]iin the same specimens. The frequency and magnitude of hypoxia-induced [Ca2+]ichanges observed in the glomus cells were enhanced in the presence of 5-HT, and this response was inhibited by the 5-HT2 receptor antagonist, ketanserin. Furthermore, RT-PCR analysis detected the expression of 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1F, 5-HT2A, 5-HT2B, 5-HT3A, and 5-HT3B receptor mRNAs in extracts of the CB. These results suggest that 5-HT increases hypoxia-induced [Ca2+]iresponses in glomus cells. 5-HT may elevate hypoxic responses in glomus cells in order to increase chemosensory activity of the CB.