Advancing animal health and disease research in the lab with three-dimensional cell culture systems.
Advancing animal health and disease research in the lab with three-dimensional cell culture systems.
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DOI：
10.1002/vetr.1528
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
David Smith;D. Price;M. Faber;A. Chapuis;T. McNeilly
中科院分区：
文献类型：
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作者：
David Smith;D. Price;M. Faber;A. Chapuis;T. McNeilly
代表兽医动物组织的三维细胞培养系统的开发正在加速寻求解决与动物健康相关的特定问题的研究。就其相关性和复杂性而言，这些体外模型可以被视为更简化的单细胞培养系统和复杂的活体动物之间的中点。特别是类器官代表了一个重要的发展，因为它们有组织的多细胞结构比以前开发的任何其他细胞培养技术更接近于体内组织。在这篇综述中，我们提供了一个概述的不同的三维细胞培养系统提供给兽医研究人员，并给出了他们的应用背景下，与动物健康的例子。
The development of three-dimensional cell culture systems representative of tissues from animals of veterinary interest is accelerating research that seeks to address specific questions tied to animal health. In terms of their relevance and complexity, these in vitro models can be seen as a midpoint between the more reductionist single-cell culture systems and complex live animals. Organoids in particular represent a significant development due to their organised multicellular structure that more closely represents in vivo tissues than any other cell culture technology previously developed. In this review, we provide an overview of the different three-dimensional cell culture systems available to veterinary researchers and give examples of their application in contexts relating to animal health.