Genetic and antigenic diversity among noroviruses
Genetic and antigenic diversity among noroviruses
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DOI：
10.1099/vir.0.81532-0
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发表时间：
2006-04-01
影响因子：
3.8
通讯作者：
Takeda, N
中科院分区：
文献类型：
--
作者：
Hansman, GS;Natori, K;Takeda, N
人类诺如病毒(NOV)株在全球范围内引起相当数量的胃肠炎暴发。根据新城疫病毒衣壳基因(VP1)的序列，可将其分为两个基因群(GI和GII)和至少14个GI和17个GIL型(GI/1-14和GII/1-17)。人类新城疫病毒株不能在细胞培养系统中繁殖，但重组VP1在昆虫细胞中的表达会导致病毒样颗粒(VLP)的形成。为了更好地了解NOV的抗原关系，对26个不同的NOV VLP进行了交叉反应分析。系统发育分析将这些新城疫病毒分为6个GI型和12个GII型。用针对这些VLP的多克隆抗血清进行抗体ELISA法检测交叉反应。抗血清与同源VLP反应强烈；然而，在不同的基因型间观察到许多新的交叉反应。例如，Gl/11抗血清表现出广泛的交叉反应，检测到两种Gi和10种Gii基因。同样，GII/1、GII/10和GII/12抗血清表现出广泛的交叉反应，检测到其他几种不同的GII基因。对VP1氨基酸序列的比对表明，这些广泛的交叉反应是由于位于外壳和/或P1-1结构域中的保守氨基酸残基。然而，不同的GII/3抗血清之间存在异常的交叉反应，这表明保守的氨基酸残基和VP1的二级结构都影响抗原性。
Human norovirus (NoV) strains cause a considerable number of outbreaks of gastroenteritis worldwide. Based on their capsid gene (VP1) sequence, human NoV strains can be grouped into two genogroups(GI and GII) and at least 14 GI and 17 Gill genotypes (GI/1-14 and GII/1-17). Human NoV strains cannot be propagated in cell-culture systems, but expression of recombinant VP1 in insect cells results in the formation of virus-like particles (VLPs). In order to understand NoV antigenic relationships better, cross-reactivity among 26 different NoV VLPs was analysed. Phylogenetic analyses grouped these NoV strains into six GI and 12 GII genotypes. An antibody ELISA using polyclonal antisera raised against these VLPs was used to determine cross-reactivity. Antisera reacted strongly with homologous VLPs; however, a number of novel cross-reactivities among different genotypes was observed. For example, Gl/11 antiserum showed a broad-range cross-reactivity, detecting two GI and 10 GII genotypes. Likewise, GII/1, GII/10 and GII/12 antisera showed a broad-range cross-reactivity, detecting several other distinct GII genotypes. Alignment of VP1 amino acid sequences suggested that these broad-range cross-reactivities were due to conserved amino acid residues located within the shell and/or P1-1 domains. However, unusual cross-reactivities among different GII/3 antisera were found, with the results indicating that both conserved amino acid residues and VP1 secondary structures influence antigenicity.