A Genome-Wide Screen in Saccharomyces cerevisiae Reveals a Critical Role for Oxidative Phosphorylation in Cellular Tolerance to Lithium Hexafluorophosphate.
A Genome-Wide Screen in Saccharomyces cerevisiae Reveals a Critical Role for Oxidative Phosphorylation in Cellular Tolerance to Lithium Hexafluorophosphate.
复制标题
酿酒酵母的全基因组筛选揭示了氧化磷酸化在细胞对六氟磷酸锂的耐受性中的关键作用
作者：
Jin X;Zhang J;An T;Zhao H;Fu W;Li D;Liu S;Cao X;Liu B
Lithium hexafluorophosphate (LiPF6) is one of the leading electrolytes in lithium-ion batteries, and its usage has increased tremendously in the past few years. Little is known, however, about its potential environmental and biological impacts. In order to improve our understanding of the cytotoxicity of LiPF6 and the specific cellular response mechanisms to it, we performed a genome-wide screen using a yeast (Saccharomyces cerevisiae) deletion mutant collection and identified 75 gene deletion mutants that showed LiPF6 sensitivity. Among these, genes associated with mitochondria showed the most enrichment. We also found that LiPF6 is more toxic to yeast than lithium chloride (LiCl) or sodium hexafluorophosphate (NaPF6). Physiological analysis showed that a high concentration of LiPF6 caused mitochondrial damage, reactive oxygen species (ROS) accumulation, and ATP content changes. Compared with the results of previous genome-wide screening for LiCl-sensitive mutants, we found that oxidative phosphorylation-related mutants were specifically hypersensitive to LiPF6. In these deletion mutants, LiPF6 treatment resulted in higher ROS production and reduced ATP levels, suggesting that oxidative phosphorylation-related genes were important for counteracting LiPF6-induced toxicity. Taken together, our results identified genes specifically involved in LiPF6-modulated toxicity, and demonstrated that oxidative stress and ATP imbalance maybe the driving factors in governing LiPF6-induced toxicity.
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影响因子：
4.8
作者：
Fontanesi, Flavia;Clemente, Paula;Barrientos, Antoni
通讯作者：
Barrientos, Antoni
影响因子：
6.4
作者：
Ye X;Morikawa K;Ho SH;Araki M;Nishida K;Hasunuma T;Hara KY;Kondo A
通讯作者：
Kondo A
影响因子：
3.9
作者：
Kalinowska, Monika;Hawrylak-Nowak, Barbara;Szymanska, Maria
通讯作者：
Szymanska, Maria
DOI：
10.1017/s1461145708009802
发表时间：
2009-07
期刊：
The international journal of neuropsychopharmacology
影响因子：
--
作者：
Bachmann RF;Wang Y;Yuan P;Zhou R;Li X;Alesci S;Du J;Manji HK
通讯作者：
Manji HK
影响因子：
46.9
作者：
通讯作者：
--