Increased expression of prolactin receptor gene assessed by quantitative polymerase chain reaction in human breast tumors versus normal breast tissues
Increased expression of prolactin receptor gene assessed by quantitative polymerase chain reaction in human breast tumors versus normal breast tissues
复制标题
DOI：
10.1210/jc.83.2.667
复制
发表时间：
1998-02-01
影响因子：
5.8
通讯作者：
Kelly, PA
中科院分区：
文献类型：
--
作者：
Touraine, P;Martini, JF;Kelly, PA
PRL在人类乳腺肿瘤发生中的作用还不清楚。限制之一是难以准确测量人体组织中的PRL受体（PRLR）。我们建立了T-47 D人乳腺癌细胞的定量PCR方法（Q-PCR），并将其应用于29例患者，其中25例患有癌症或纤维腺瘤。4例患者接受了乳房成形术，在Q-PCR前培养正常上皮细胞。在T-47 D细胞中，每微克总RNA检测到31 × 106个信使RNA分子。在所有患者中，检测到PRLR基因的表达，在正常组织中从1500到1 × 10（6）分子/μ g RNA变化，在肿瘤中从4500到34.7 × 10（6）分子/μ g RNA变化。PRLR在肿瘤中的表达高于正常乳腺组织和邻近组织，在培养的乳腺上皮细胞和正常乳腺组织中的表达相似。雌激素和孕激素受体阴性的肿瘤表达低水平的催乳素受体转录本，类似于正常的乳腺组织从绝经期妇女。PRLR的免疫细胞化学分析证实，与正常组织相比，几乎所有的肿瘤样品中的染色更强。还通过RT-PCR在每个测试样品中鉴定了编码本地产生的人PRL的信使RNA。我们的研究结果证实了PRLR基因在所有研究组织中的表达，而且，表明这种表达在人类乳腺肿瘤中比正常相邻组织中增加。
The role of PRL in human breast tumorigenesis is not well understood. One of the limitations is the difficulty of accurately measuring PRL receptors (PRLR) in human tissues. We established a quantitative PCR method (Q-PCR) in T-47D human breast cancer cells and applied it to 29 patients, 25 of whom presented with either cancer or fibroadenoma. Four patients underwent a mammoplasty, and normal epithelial cells were cultured before Q-PCR. In T-47D cells, 31 x 10(6) messenger RNA molecules were detected per microgram of total RNA. In all patients, expression of the PRLR gene was detected, varying from 1500 to 1 x 10(6) molecules/mu g of RNA in normal tissues and from 4500 to 34.7 x 10(6) molecules/mu g of RNA in tumors. PRLR expression was always greater in tumor than in normal;contiguous tissue and similar in cultured mammary epithelial cells and normal breast tissues. Estradiol and progesterone receptor-negative tumors expressed low levels of PRLR transcripts, similar to normal breast tissue from menopausal women. Immunocytochemical analysis of PRLR confirmed stronger staining in almost all tumor samples compared with normal tissues. A messenger RNA encoding locally produced human PRL was also identified by RT-PCR in every sample tested. Our results confirm PRLR gene expression in all tissues studied, and moreover, indicate that this expression is increased in human breast tumors vs. normal contiguous tissues.