The fractionation of adipose tissue procedure to obtain stromal vascular fractions for regenerative purposes
The fractionation of adipose tissue procedure to obtain stromal vascular fractions for regenerative purposes
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DOI：
10.1111/wrr.12482
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发表时间：
2016-09
影响因子：
2.9
通讯作者：
J. V. van Dongen;H. Stevens;Mojtaba Parvizi;B. van der Lei;M. Harmsen
中科院分区：
文献类型：
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作者：
J. V. van Dongen;H. Stevens;Mojtaba Parvizi;B. van der Lei;M. Harmsen
自体脂肪组织移植在临床上用于减少真皮疤痕并恢复体积损失。对组织损伤的治疗效果更可能取决于脂肪组织的基质血管部分而不是脂肪细胞部分。该基质血管部分可以通过酶解或机械解离脂肪组织来获得。酶解过程既耗时又昂贵。因此，我们开发了一种新的廉价机械分离程序，以节省时间的方式从脂肪组织中获得基质血管部分，可直接用于治疗注射。这种机械分离过程被称为脂肪组织分离（FAT）过程。 11 名患者接受了 FAT 手术。通过免疫组织化学对 FAT 基质血管部分的组成进行了表征。将从 FAT 基质血管部分分离的脂肪源性基质细胞与从非解离脂肪组织（对照）分离的脂肪源性基质细胞的 CD 表面标记物表达、分化和集落形成单位能力进行比较。病例报告证明了 FAT 基质血管成分的治疗效果。 FAT 基质血管部分是细胞外基质的富集，含有微血管和可培养的脂肪衍生基质细胞。从 FAT 基质血管部分分离的脂肪源性基质细胞与从对照组分离的脂肪源性基质细胞在 CD 表面标记物表达、分化和集落形成单位能力方面没有差异。 FAT 程序是一种快速有效的机械解离程序，可生成 FAT 基质血管部分，以便与脂肪组织的所有治疗成分一起注射：它包含可培养的脂肪衍生基质细胞，与微血管系统一起嵌入其天然支持性细胞外基质中。
Autologous adipose tissue transplantation is clinically used to reduce dermal scarring and to restore volume loss. The therapeutic benefit on tissue damage more likely depends on the stromal vascular fraction of adipose tissue than on the adipocyte fraction. This stromal vascular fraction can be obtained by dissociation of adipose tissue, either enzymatically or mechanical. Enzymatic dissociation procedures are time‐consuming and expensive. Therefore, we developed a new inexpensive mechanical dissociation procedure to obtain the stromal vascular fraction from adipose tissue in a time sparing way, which is directly available for therapeutic injection. This mechanical dissociation procedure is denoted as the fractionation of adipose tissue (FAT) procedure. The FAT procedure was performed in eleven patients. The composition of the FAT‐stromal vascular fraction was characterized by immunohistochemistry. Adipose derived stromal cells isolated from the FAT‐stromal vascular fraction were compared with adipose derived stromal cells isolated from nondissociated adipose tissue (control) for their CD‐surface marker expression, differentiation and colony forming unit capacity. Case reports demonstrated the therapeutic effect of the FAT‐stromal vascular fraction. The FAT‐stromal vascular fraction is an enrichment of extracellular matrix containing a microvasculature and culturable adipose derived stromal cells. Adipose derived stromal cells isolated from FAT‐stromal vascular fraction did not differ from adipose derived stromal cells isolated from the control group in CD‐surface marker expression, differentiation and colony forming unit capacity. The FAT procedure is a rapid effective mechanical dissociation procedure to generate FAT‐stromal vascular fraction ready for injection with all its therapeutic components of adipose tissue: it contains culturable adipose derived stromal cells embedded in their natural supportive extracellular matrix together with the microvasculature.