Magnetic solid-phase extraction of four β-lactams using polypyrrole-coated magnetic nanoparticles from water samples by micellar electrokinetic capillary chromatography analysis
Magnetic solid-phase extraction of four β-lactams using polypyrrole-coated magnetic nanoparticles from water samples by micellar electrokinetic capillary chromatography analysis
复制标题
通过胶束电动毛细管色谱分析，使用聚吡咯涂覆的磁性纳米粒子从水样中磁性固相萃取四种 β-内酰胺
DOI：
10.1016/j.chroma.2019.460541
复制
发表时间：
2020-01-11
影响因子：
4.1
通讯作者：
Li, Zengning
中科院分区：
文献类型：
--
作者：
Li, Xinghua;Yin, Zhendong;Li, Zengning
在本研究中，聚吡咯涂层的Fe 3 O 4磁性纳米粒子（PPy/Fe 3 O 4 MNP）作为吸附剂的应用得到证实。采用TEM、FTIR、XRD等手段对复合材料进行了表征。选择四种β-内酰胺（苯唑西林（OXA）、氯唑西林（CLOX）、双氯西林（DIC）和氟氯西林（FLU））作为实验的分析物。采用胶束电动毛细管色谱-二极管阵列检测器（MEKC-DAD）测定β-内酰胺类抗生素。对影响萃取效率和分离效果的关键参数进行了研究和优化。在最佳条件下，检测限为1.0 μ g L-1的OXA，CLOX，FLU和0.8 μ g L-1的DIC。线性范围为2.5-200.0 μ g L ~（-1）。方法用于水样中β-内酰胺类抗生素的测定，结果满意。日内和日间相对标准偏差分别为1.09%~ 4.58%和2.95%~ 7.80%。结果表明，该方法灵敏、简便、可行，可用于水样中β-内酰胺类抗生素的测定。(C)2019爱思唯尔B. V.保留所有权利。
In the present research, the application of a polypyrrole coating on Fe3O4 magnetic nanoparticles (PPy/Fe3O4 MNPs) as a sorbent was confirmed. The synthesized magnetic composites were characterized by TEM, FTIR spectroscopy, and XRD. Four beta-lactams (oxacillin (OXA), cloxacillin (CLOX), dicloxacillin (DIC), and flucloxacillin (FLU)) were selected as analytes for the experiment. The extracted beta-lactams were determined by micellar electrokinetic capillary chromatography-diode array detector (MEKC-DAD). The crucial parameters influencing the extraction efficiency and separation were studied and optimized. Under the optimal conditions, the limits of detection are 1.0 mu g L-1 for OXA, CLOX, FLU, and 0.8 mu g L-1 for DIC. The calibration curves are linear in the range of 2.5-200.0 mu g L-1. The proposed method was applied for the determination of the beta-lactams in water samples with satisfactory results. The intra-day relative standard deviations and the inter-day relative standard deviations range from 1.09% to 4.58% and from 2.95% to 7.80%, respectively. It can be concluded that this method is sensitive, convenient, and feasible for the determination of the beta-lactams in water samples. (C) 2019 Elsevier B.V. All rights reserved.