Age-related changes in the subsets and functions of human T lymphocytes.
Age-related changes in the subsets and functions of human T lymphocytes.
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DOI：
10.4049/jimmunol.121.5.1773
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发表时间：
1978-11
影响因子：
4.4
通讯作者：
S. Kishimoto;S. Tomino;K. Inomata;S. Kotegawa;T. Saito;M. Kuroki;H. Mitsuya;S. Hisamitsu
中科院分区：
文献类型：
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作者：
S. Kishimoto;S. Tomino;K. Inomata;S. Kotegawa;T. Saito;M. Kuroki;H. Mitsuya;S. Hisamitsu
本研究的目的是通过使用各种免疫学参数来研究与人类衰老相关的免疫活性的变化。在这项研究中检查的志愿者是健康的或表面上健康的，没有影响免疫系统的疾病，年龄从14岁到89岁不等。本研究的结果总结见表I。外周血淋巴细胞（PBL）绝对数随增龄而下降。成人组（60岁以下）的平均计数为2043，老年组（60岁以上）的平均计数为1659。当在4°C下孵育1小时后，在存在胎牛血清的情况下，用神经氨酸酶处理的绵羊红细胞通过玫瑰花结形成来测定T淋巴细胞时，组间T淋巴细胞的比例没有差异，而在不存在胎牛血清的情况下，用绵羊红细胞通过玫瑰花结形成来测定的T细胞的比例在老年组中显著降低（p < 0.01）。另一方面，在室温下以1500 RPM离心5 min后，用绵羊红细胞玫瑰花结法测定的T淋巴细胞比例，老年组高于成年组（p < 0.02）。用羊红细胞和兔IgG抗体致敏的鸡红细胞双玫瑰花结法检测，老年组携带IgG-Fc受体的T淋巴细胞比例也高于成年组（p < 0.05）。补体受体携带淋巴细胞的比例没有随着年龄的增长而变化。此外，在pH 4.0醋酸盐缓冲液中处理1分钟以从细胞表面除去非特异性结合的IG后，检测的表面Ig携带淋巴细胞的比例在两组之间没有差异。老年组（70 - 83岁）对PHA和伴刀豆球蛋白A的反应低于年轻组（20 - 40岁）（p < 0.01），而对美洲商陆有丝分裂原的反应则相同。PHA反应与携带IgG-Fc受体的T细胞的比例呈负相关。与丝裂霉素处理的伯基特淋巴瘤来源的AL 1细胞的混合淋巴细胞培养反应在老年组中也受损（p < 0.01），细胞介导的对作为靶细胞的AL 1细胞的细胞毒性也受损（p < 0.05）。老年组血浆IgG、伊加水平显著高于成年组（P < 0.001），而IgM、IgE水平无显著差异。IG类的放射免疫测定显示，当PBL在PWM存在下培养7天时，老年人体外合成的IgG的几何平均值显著高于青年人组（p < 0.05），而IgM或伊加的几何平均值则不显著。老年组IgG合成的增加归因于辅助性T细胞活性的增强。
The purpose of this study was to investigate changes in immunocompetence associated with aging in humans by the use of various immunologic parameters. Volunteers examined in this study were healthy or apparently healthy without diseases affecting the immune system and ranged from 14 to 89 years of age. The results of this study are summarized in Table I. The absolute number of peripheral blood lymphocytes (PBL) decreased with aging. The mean count was 2043 in the adult group (less than 60 years of age) and 1659 in the aged group (over 60 years of age). When T lymphocytes were assayed by rosette formation with neuraminidase-treated sheep erythrocytes in the presence of fetal calf serum after 1 hr incubation at 4°C, there was no difference in the proportions of T lymphocytes between the groups, whereas the proportions of T cells as assayed by rosette formation with sheep erythrocytes in the absence of fetal calf serum were significantly depressed in the aged group (p < 0.01). On the other hand, the proportion of T lymphocytes assayed by rosette formation with sheep erythrocytes after centrifugation at 1500 RPM for 5 min at room temperature was higher in the aged group than in the adult group (p < 0.02). The proportion of IgG-Fc receptor-bearing T lymphocytes detected by double rosette formation with sheep erythrocytes and chicken erythrocytes sensitized with rabbit IgG antibody was also higher in the aged than in the adult group (p < 0.05). The proportion of complement receptor-bearing lymphocytes did not change with aging. In addition, the proportion of surface Ig-bearing lymphocytes examined after being treated in pH 4.0 acetate buffer for 1 min to remove nonspecifically bound Ig from the cell surface showed no differences between the two groups. Response to PHA and concanavalin A was impaired in the aged (aged 70 to 83 years) compared to the young adult group (aged 20 to 40 years) (p < 0.01), whereas the response to pokeweed mitogen was of the same magnitude. PHA response was negatively correlated with the proportion of IgG-Fc receptor-bearing T cells. The mixed lymphocyte culture reaction with mitomycin-treated AL1 cells derived from Burkitt's lymphoma was also impaired in the aged group (p < 0.01) as was cell-mediated cytotoxicity to AL1 cells as target cells (p < 0.05). Levels of IgG and IgA in plasma were significantly higher in the aged than in the adult group (p < 0.001), whereas those of IgM and IgE were not different. Radioimmunoassay determination of Ig classes revealed that the geometric means of IgG, but not IgM or IgA, synthesized in vitro was significantly increased in the aged compared to the young adult group (p < 0.05) when PBL were cultured in the presence of PWM for 7 days. This increase in IgG synthesis in the aged group was attributed to enhanced T helper activity.