Invariant NKT cells sustain specific B cell responses and memory
Invariant NKT cells sustain specific B cell responses and memory
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DOI：
10.1073/pnas.0700191104
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发表时间：
2007-03-06
影响因子：
11.1
通讯作者：
Abrignani, Sergio
中科院分区：
文献类型：
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作者：
Galli, Grazia;Pittoni, Paola;Abrignani, Sergio
不变自然杀伤T细胞（iNKT）是一种先天性淋巴细胞，可识别cdd限制性糖脂抗原，如α -半乳糖神经酰胺（α GC）。我们评估了iNKT细胞是否有助于B淋巴细胞反应，发现用蛋白质和aGC免疫的小鼠产生的抗体滴度比单独由蛋白质诱导的小鼠高1-2倍。iNKT细胞的激活增强了对流感等感染的保护，并引起记忆B细胞频率更高，对加强免疫的抗体反应更高。在缺乏MHC 11类分子的小鼠中，用α - GC而不是传统佐剂接种蛋白疫苗可引起IgG反应，这表明iNKT细胞可以替代CD4(+) T细胞帮助B细胞。有趣的是，在缺乏iNKT细胞的小鼠中，循环抗体的衰减速度更快。这些发现表明iNKT细胞在抗体反应的关键特征（如免疫和B细胞记忆）中具有稳态作用。
Invariant natural killer T (iNKT) cells are innate-like lymphocytes recognizing CDid-restricted glycolipid antigens, such as alpha-galactosylceramide (alpha GC). We assessed whether iNKT cells help B lymphocyte responses and found that mice immunized with proteins and aGC develop antibody titers 1-2 logs higher than those induced by proteins alone. Activation of iNKT cells enhances protection against infections such as influenza and elicits higher frequencies of memory B cells and higher antibody responses to booster immunizations. Protein vaccination with alpha GC, but not with conventional adjuvants, elicits IgG responses in mice lacking MHC class 11 molecules, demonstrating that iNKT cells can substitute for CD4(+) T cell help to B cells. Interestingly, the decay of circulating antibodies is faster in mice lacking iNKT cells. These findings point to a homeostatic role for iNKT cells on critical features of the antibody response such as immunity and B cell memory.