Chemokine CCL20 enhances the growth of HuH7 cells via phosphorylation of p44/42 MAPK in vitro
Chemokine CCL20 enhances the growth of HuH7 cells via phosphorylation of p44/42 MAPK in vitro
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DOI：
10.1016/j.bbrc.2004.07.207
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发表时间：
2004-09-24
影响因子：
3.1
通讯作者：
Okanoue, T
中科院分区：
文献类型：
--
作者：
Fujii, H;Itoh, Y;Okanoue, T
CCR6是趋化因子CCL20的受体。在本研究中，我们证明了CCR6在人HCC细胞系（HuH7、PLC/PRF/5和HepG2）上的表面表达增强，特别是在HuH7细胞上，而在HLE和HLF细胞上没有。这些HCC细胞系（HuH7、PLC/PRF/5和HepG2）特别是HuH7细胞自发分泌大量CCL20，而HLE或HLF细胞则没有。CCL20刺激HuH7细胞上调CCR6 mRNA表达，显著促进HuH7细胞生长。ccl20刺激的HuH7细胞生长被p44/42 MAPK介导的下游信号转导通路抑制所消除，但p38 MAPK或SAPK/JNK不受影响。RT-PCR证实CCR6在人肝癌组织中的表达。这些结果表明，一部分人HCC细胞的生长可能像HuH7细胞一样，由CCL20-CCR6轴以自分泌或旁分泌的方式介导。(C) 2004爱思唯尔公司版权所有。
CCR6 is the receptor of chemokine CCL20. In the present study, we demonstrated that the surface expression of CCR6 was enhanced on the human HCC cell lines (HuH7, PLC/PRF/5, and HepG2) especially on HuH7 cells, but not on HLE or HLF Cells. These HCC cell lines (HuH7, PLC/PRF/5, and HepG2) especially the HuH7 cells secreted a significant amount of CCL20 spontaneously, whereas HLE or HLF did not. Stimulation by CCL20 up-regulated the mRNA expression of CCR6 in HuH7 cells and significantly enhanced the growth of HuH7 cells. CCL20-stimulated growth of HuH7 cells was abrogated by the inhibition of downstream signal transduction pathway mediated by p44/42 MAPK, but not by p38 MAPK or SAPK/JNK. CCR6 expression in human HCC tissues was confirmed by RT-PCR. These results indicate that the growth of a proportion of human HCC cells may be mediated by CCL20-CCR6 axis, like HuH7 cells, in an autocrine or paracrine manner. (C) 2004 Elsevier Inc. All rights reserved.