THE FATE OF LABELED GLUCOSE MOLECULES IN THE RAT ADIPOCYTE - DEPENDENCE ON GLUCOSE-CONCENTRATION
THE FATE OF LABELED GLUCOSE MOLECULES IN THE RAT ADIPOCYTE - DEPENDENCE ON GLUCOSE-CONCENTRATION
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DOI：
10.1016/0167-4889(84)90100-9
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
FOLEY, JA
中科院分区：
文献类型：
--
作者：
GLIEMANN, J;REES, WD;FOLEY, JA
Isolated rat adipocytes were incubated with 15 nM [3-3H]glucose or 100 nM [U-14C]glucose with or without insulin and in the absence or presence of unlabeled glucose. Following a 2 h incubation with 15 nM [3-3H]glucose, about 2/3 of the cell-associated 3H-labeled metabolic products were hydrophilic largely anionic intermediates and about 1/3 was lipids. The equivalent values were 40 and 60%, respectively, when using 100 nM [U-14C]glucose. The only 14C-labeled metabolite escaping to the incubation medium was 14CO2, which accounted for about 15% of the rate of metabolism. The rate of incorporation of 100 nM [U-14C]glucose into the cell-associated metabolites was quite a good measure of its net influx rate. The conversion of the 2 tracers to the sum of the metabolic products in cells treated with a maximally stimulating insulin concentration remained constant with glucose concentrations up to about 100 .mu.M and then decreased progressively. The incorporation of radioactivity into the different metabolites varied markedly over the glucose concentration range 0-100 .mu.M, presumably due to the saturation of different metabolic pools at different glucose concentrations. This variation was much less in cells not stimulated with insulin. Consequently, the maximal effect of insulin on the incorporation of the tracers into a given metabolite (e.g., labeled lipids) varied over the entire glucose concentration range. The apparent sensitivity (ED50) with respect to the incorporation into a given metabolite was also dependent on the glucose concentration.
