Inheritance patterns of transcript levels in F1 hybrid mice
Inheritance patterns of transcript levels in F1 hybrid mice
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DOI：
10.1534/genetics.106.060251
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发表时间：
2006-10-01
期刊：
影响因子：
3.3
通讯作者：
Churchill, Gary A.
中科院分区：
文献类型：
--
作者：
Cui, Xiangqin;Affourtit, Jason;Churchill, Gary A.
转录调控的遗传分析是一个迅速兴起的研究领域，有望揭示控制基因表达的调控网络。虽然已经进行了一些这样的研究，基因表达性状的遗传性的性质和程度还没有得到很好的确定。我们描述了遗传的转录水平在肝脏组织中的第一个子（F-1）代的小鼠从常见的近交系A/J和C57 BL/6 J之间的相互杂交。我们从这些数据中获得遗传和技术方差分量的估计，并证明收缩估计可以增加可检测的遗传力。遗传力的估计值在不同的转录本之间差异很大，三分之一的转录本基本上没有遗传力（< 0.01），四分之一的转录本显示出非常高的遗传力（> 0.50）。大约一半的转录本在两个亲本菌株之间差异表达。大多数转录本显示出加性遗传模式。显性效应观察到20%的成绩单和少量的成绩单被确定为表现出超显性遗传模式。此外，我们确定了314个转录本的表达水平不同的相互F-1动物。这些基因可能与母体效应有关。
Genetic analysis of transcriptional regulation is a rapidly emerging field of investigation that promises to shed light on the regulatory networks that control gene expression. Although a number of such studies have been carried out, the nature and extent of the heritability of gene expression traits have not been well established. We describe the inheritance of transcript levels in liver tissues in the first filial (F-1) generation of mice obtained from reciprocal crosses between the common inbred strains A/J and C57BL/6J. We obtain estimates of genetic and technical variance components from these data and demonstrate that shrinkage estimators can increase detectable heritability. Estimates of heritability vary widely from transcript to transcript, with one-third of transcripts showing essentially no heritability (< 0.01) and one-quarter showing very high heritability (> 0.50). Roughly half of all transcripts are differentially expressed between the two parental strains. Most transcripts show an additive pattern of inheritance. Dominance effects were observed for 20% of transcripts and a small number of transcripts were identified as showing an overdominance mode of inheritance. In addition, we identified 314 transcripts with expression levels that differ between the reciprocal F-1 animals. These genes may be related to maternal effect.