Monocyte responsiveness to chemotactic stimuli is a property of a subpopulation of cells that can respond to multiple chemoattractants.
Monocyte responsiveness to chemotactic stimuli is a property of a subpopulation of cells that can respond to multiple chemoattractants.
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单核细胞对趋化刺激的反应性是可以对多种趋化剂作出反应的细胞亚群的特性。
DOI：
10.1172/jci110033
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Snyderman,R
中科院分区：
文献类型：
--
作者：
Cianciolo,GJ;Snyderman,R
在白细胞的趋化迁移之前，细胞的形状会从圆形转变为特有的极型。我们已经开发了一种分析方法，表明当人单核细胞暴露在悬浮液中的化学诱导剂时，会呈现这种极化的形状。所研究的三种化学诱导剂，趋化淋巴因子，补体激活的血清，然后甲酰化的寡肽，都以时间、温度和剂量依赖的方式激发极化。非趋化性药物，如丝裂原或佛波酯醋酸酯，不会引起极化。在37℃时，极化迅速(1分钟)，可被细胞松弛素B、叠氮化钠或低温抑制。对一系列N-甲酰化低聚肽进行了研究，发现它们的诱导极化活性与趋化活性密切相关(r>；0.99)。在循环单核细胞的整个群体中，有一群细胞能够对趋化刺激做出反应而极化。单核细胞对任何趋化因子极化的最大百分比为∼60%。此外，几种趋化因子的组合不能增加极化单核细胞的百分比，超过任何单一化学诱导剂的最佳剂量所获得的最大百分比。数据还表明，高剂量的化学诱导剂可以在单核细胞中诱导交叉脱敏状态，阻止细胞对其他类型的趋化因子的反应。这些结果支持这样的概念，即对趋化刺激有反应的单核细胞能够对几种吸引物中的任何一种产生反应。
The chemotactic migration of leukocytes is preceded by an alteration in the cells' shape from round to a characteristic polar configuration. We have developed an assay that shows that human monocytes, when exposed to chemoattractant in suspension, assume this polarized shape. The three types of chemo-attractants studied, a chemotactic lymphokine, complement-activated serum, and theN-formylated oligopeptides, all induced polarization in a time, temperature, and dose-dependent fashion. Nonchemotactic agents such as mitogens or phorbol myristate acetate did not induce polarization. At 37°C, polarization was rapid (<1 min) and was inhibitable by cytochalasin B, sodium azide, or low temperature. A series ofN-formylated oligopeptides were studied and their activity in inducing polarization correlated closely (r> 0.99) with their chemotactic activity. Of the entire population of circulating monocytes there is a subpopulation of cells that is capable of polarizing in response to chemotactic stimuli. The maximum percentage of monocytes which polarized to any chemotactic factor was ∼60%. Furthermore, the combination of several chemotactic factors could not increase the percentage of polarized monocytes above the maximum obtained with an optimal dose of any single chemoattractant. The data also demonstrate that high doses of a chemoattractant can induce a state of cross-desensitization in monocytes that blocks the response of the cells to other types of chemotactic factors. These results support the concept that the monocytes that do respond to chemotactic stimuli are capable of responding to any of several attractants.Images