A nuclear phylogenomic study of the angiosperm order Myrtales, exploring the potential and limitations of the universal Angiosperms353 probe set
A nuclear phylogenomic study of the angiosperm order Myrtales, exploring the potential and limitations of the universal Angiosperms353 probe set
复制标题
被子植物目桃金娘目的核系统发育学研究，探索通用被子植物353探针组的潜力和局限性
DOI：
10.1002/ajb.1699
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发表时间：
2021
影响因子：
3
通讯作者：
Maurin, O.
中科院分区：
文献类型：
--
作者：
Maurin, O.
为了进一步加深对分布于各大洲(除南极洲外)的9科约400属近14,000种被子植物目Myrtales的了解，我们测试了被子植物353探针试剂盒。方法我们结合高通量测序和靶向富集法与Angiosperms353探针试剂盒相结合，对305属485种(占该目所有属的76%)的样本进行了评估。所有级别的关系，例如早期分裂的家庭的关系，往往反映了之前的研究，但基因冲突是明显的，以前被发现不确定的关系往往仍然如此。结论高通量测序和Angiosperms353探针试剂盒是系统学分析的有力工具，但需要更好地理解现有的遗传数据，以确定可能导致谱系分类和/或杂交事件不完整的基因和基因树。
PremiseTo further advance the understanding of the species‐rich, economically and ecologically important angiosperm order Myrtales in the rosid clade, comprising nine families, approximately 400 genera and almost 14,000 species occurring on all continents (except Antarctica), we tested the Angiosperms353 probe kit.MethodsWe combined high‐throughput sequencing and target enrichment with the Angiosperms353 probe kit to evaluate a sample of 485 species across 305 genera (76% of all genera in the order).ResultsResults provide the most comprehensive phylogenetic hypothesis for the order to date. Relationships at all ranks, such as the relationship of the early‐diverging families, often reflect previous studies, but gene conflict is evident, and relationships previously found to be uncertain often remain so. Technical considerations for processing HTS data are also discussed.ConclusionsHigh‐throughput sequencing and the Angiosperms353 probe kit are powerful tools for phylogenomic analysis, but better understanding of the genetic data available is required to identify genes and gene trees that account for likely incomplete lineage sorting and/or hybridization events.