PROSTAGLANDIN-E (PGE) AND INTERLEUKIN-1-BETA (IL-1-BETA) LEVELS IN GINGIVAL CREVICULAR FLUID DURING HUMAN ORTHODONTIC TOOTH MOVEMENT
PROSTAGLANDIN-E (PGE) AND INTERLEUKIN-1-BETA (IL-1-BETA) LEVELS IN GINGIVAL CREVICULAR FLUID DURING HUMAN ORTHODONTIC TOOTH MOVEMENT
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DOI：
10.1016/s0889-5406(94)70131-8
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发表时间：
1994-04-01
影响因子：
3
通讯作者：
DUBOIS, LM
中科院分区：
文献类型：
--
作者：
GRIEVE, WG;JOHNSON, GK;DUBOIS, LM
本研究的目的是检测人类正畸牙齿移动过程中两种有效骨吸收介质前列腺素 E (PGE) 和白介素-1β (IL-1beta) 的龈沟液 (GCF) 水平。该研究包括 10 名患者，每名患者都有一颗正在接受正畸移动的治疗牙齿和一颗对侧对照牙齿。在激活前以及激活后 1、24、48 和 168 小时在对照部位和治疗（压缩）部位对 GCF 进行采样。预防斑块引起的炎症使这项研究能够重点关注机械刺激的 PGE 和 IL-1beta GCF 水平的动态。采用放射免疫法测定PGE和IL-1β水平。在第 1 小时和第 24 小时，与对照牙齿（分别为 2.0 +/- 1.1 pg，p = 0.0049 和 2.9 - 1.0 pg，p = 0.0209）相比，治疗牙齿的平均 GCF IL-1β 水平显着升高（分别为 8.9 +/- 2.0 和 19.2 +/- 6.0 pg）。治疗牙齿的 PGE 的 GCF 水平在 24 小时和 48 小时（108.9 +/- 11.9 和 97.9 +/- 7.3 pg）显着高于对照牙齿（分别为 61.8 +/- 7.2 pg，p = 0.0071 和 70.8 +/- 7.4 pg，p = 0.0021）。在整个研究过程中，对照牙齿的 GCF 水平 PGE 和 IL-1β 保持在基线水平，而随着时间的推移，在治疗牙齿中观察到 GCF IL-1β（24 小时）和 PGE 水平（24 小时和 48 小时）较基线显着升高（p 小于或等于 0.05）。这些结果表明，在牙齿移动的早期阶段，GCF 中可以检测到牙周组织内产生的骨吸收 PGE 和 IL-1β，并在 7 天内恢复到基线。
The purpose of this study was to examine gingival crevicular fluid (GCF) levels of two potent bone resorbing mediators, prostaglandin E (PGE) and interleukin-1beta (IL-1beta), during human orthodontic tooth movement. The study included 10 patients, each having one treatment tooth undergoing orthodontic movement and a contralateral control tooth. The GCF was sampled at control sites and treatment (compression) sites before activation and at 1, 24, 48, and 168 hours. Prevention of plaque-induced inflammation allowed this study to focus on the dynamics of mechanically stimulated PGE and IL-1beta GCF levels. The PGE and IL-1beta levels were determined with radioimmunoassay. At 1 and 24 hours, mean GCF IL-1beta levels were significantly elevated at treatment teeth (8.9 +/- 2.0 and 19.2 +/- 6.0 pg, respectively) compared with control teeth (2.0 +/- 1.1 pg, p = 0.0049, and 2.9 - 1.0 pg, p = 0.0209, respectively). The GCF levels of PGE for the treatment teeth were significantly higher at 24 and 48 hours (108.9 +/- 11.9 and 97.9 +/- 7.3 pg) than the control teeth (61.8 +/- 7.2 pg, p = 0.0071, and 70.8 +/- 7.4 pg, p = 0.0021, respectively). The GCF levels of PGE and IL-1beta remained at baseline levels throughout the study for the control teeth, whereas significant elevations from baseline in GCF IL-1beta (24 hours) and PGE levels (24 and 48 hours) were observed over time in the treatment teeth (p less-than-or-equal-to 0.05). These results demonstrate that bone-resorbing PGE and IL-1beta produced within the periodontium are detectable in GCF during the early phases of tooth movement and return to baseline within 7 days.