The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X
The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X
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DOI：
10.1038/35057165
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发表时间：
2001-02-15
期刊：
影响因子：
64.8
通讯作者：
Wright, CL
中科院分区：
文献类型：
--
作者：
Bentley, DR;Deloukas, P;Wright, CL
我们构建了8条染色体（1、6、9、10、13、20、X和（先前的）22）的图谱，代表了三分之一的基因组，方法是建立地标图谱，分离细菌克隆和组装contigs。通过这种方法，我们可以在项目的早期建立地图的长期组织，并且所有的配置扩展、缺口闭合和问题解决都通过局部区域的遏制来简化。这些图谱目前代表了176组染色体中94%以上的常染色质（含基因）区域，并包含了人类基因图谱中96%的染色体特异性标记。通过测量剩余的间隙，我们可以评估测序克隆的染色体长度和覆盖范围。
We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.