A community outbreak of food-borne small round-structured virus gastroenteritis caused by a contaminated water supply
A community outbreak of food-borne small round-structured virus gastroenteritis caused by a contaminated water supply
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因供水受污染引起的食源性小圆结构病毒胃肠炎社区暴发
DOI：
10.1017/s0950268898001885
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发表时间：
1999
影响因子：
4.2
通讯作者：
A. Mukerjee
中科院分区：
文献类型：
--
作者：
R. Brugha;I. Vipond;M. Evans;Q. Sandifer;R. Roberts;R. Salmon;E. Caul;A. Mukerjee
1994年8月，135名面包店员工中的30人（23%）和联合王国南威尔士和布里斯托尔的100多人受到肠胃炎爆发的影响。对员工和三个社区群集的流行病学研究发现，员工的疾病与在面包店喝冷水有关（相对风险为3.3,95%，CI为1.6 - 7.0），在社区病例中，与从一家特定面包店供应的各种商店吃蛋奶冻片有关（相对风险为19.8,95%，CI为2.9 - 135.1）。在4例员工和7例社区病例的粪便标本中检出小圆结构病毒（SRSV）。利用逆转录聚合酶链反应（RT-PCR）对SRSV基因组的聚合酶和衣壳区进行分析，发现两个基因组（1和2）的病毒各自具有几种不同的核苷酸序列。在疫情中发现的病毒的异质性表明，干蛋奶冻混合物可能在无意中被污染的水重新配制。这一事件显示了次生食品污染如何导致大规模社区胃肠炎暴发，并证明了分子技术在疫情调查中支持经典流行病学方法的能力。
In August 1994, 30 of 135 (23%) bakery plant employees and over 100 people from South Wales and Bristol in the United Kingdom, were affected by an outbreak of gastroenteritis. Epidemiological studies of employees and three community clusters found illness in employees to be associated with drinking cold water at the bakery (relative risk 3·3, 95%, CI 1·6–7·0), and in community cases with eating custard slices (relative risk 19·8, 95%, CI 2·9–135·1) from a variety of stores supplied by one particular bakery. Small round-structured viruses (SRSV) were identified in stool specimens from 4 employees and 7 community cases. Analysis of the polymerase and capsid regions of the SRSV genome by reverse transcription-polymerase chain reaction (RT-PCR) demonstrated viruses of both genogroups (1 and 2) each with several different nucleotide sequences. The heterogeneity of the viruses identified in the outbreak suggests that dried custard mix may have been inadvertently reconstituted with contaminated water. The incident shows how secondary food contamination can cause wide-scale community gastroenteritis outbreaks, and demonstrates the ability of molecular techniques to support classical epidemiological methods in outbreak investigations.