DOUBLE-ANTIBODY RADIOIMMUNOASSAY FOR SERUM PROGESTERONE USING PROGESTERONE-3-(O-CARBOXYMETHYL)OXIMINO-[I-125]-IODOHISTAMINE AS RADIOLIGAND
DOUBLE-ANTIBODY RADIOIMMUNOASSAY FOR SERUM PROGESTERONE USING PROGESTERONE-3-(O-CARBOXYMETHYL)OXIMINO-[I-125]-IODOHISTAMINE AS RADIOLIGAND
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DOI：
10.1016/0039-128x(78)90052-1
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发表时间：
1978-01-01
期刊：
影响因子：
2.7
通讯作者：
MISHELL, DR
中科院分区：
文献类型：
--
作者：
SCOTT, JZ;STANCZYK, FZ;MISHELL, DR
建立了一种可靠、方便、经济的人血清黄体酮放射免疫测定法（RIA）。该方法采用乙醚提取，然后用兔抗-11 - α进行RIA。-羟孕酮-11 -半琥珀酰-牛血清白蛋白（孕酮-11. α .- bsa）血清，孕酮-3-（o-羧甲基）肟胺-[125I]-碘组胺（孕酮-3-[125I]）作为放射性配体与山羊抗兔。球蛋白作为第二抗体。和抗黄体酮一起使用。- bsa血清中，孕酮-3-[125I] RIA的总体检测特异性与使用葡聚糖包被木炭的[3H]-孕酮法相似。孕酮-3-[125I] RIA在月经周期中获得的血清孕酮测量结果与使用类似的抗孕酮-11 - α的[3H]-孕酮测定结果相似。bsa血清。然而，孕酮-3-[125I]双抗体RIA法比[3H]-孕酮RIA法更方便、更便宜。
A reliable, convenient and economical radioimmunoassay (RIA) for [human] serum progesterone was established and tested. This procedure employs diethyl ether extraction followed by RIA utilizing rabbit anti-11.alpha.-hydroxyprogesterone 11-hemisuccinyl-bovine serum albumin (progesterone-11.alpha.-BSA) serum, progesterone-3-(O-carboxymethyl)oximino-[125I]-iodohistamine (progesterone-3-[125I]) as radioligand and goat anti-rabbit .gamma. globulin as 2nd antibody. In conjunction with anti-progesterone-11.alpha.-BSA serum, the overall assay specificity of the progesterone-3-[125I] RIA is similar to that of the [3H]-progesterone method using dextran-coated charcoal. The results of serum progesterone measurements during the menstrual cycle obtained by the progesterone-3-[125I] RIA appear comparable to those of [3H]-progesterone assays which employ similar anti-progesterone-11.alpha.-BSA sera. The progesterone-3-[125I] double-antibody RIA, however, is more convenient and less expensive than the [3H]-progesterone RIA method.