U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing
U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing
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DOI：
10.1093/nar/gkp050
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发表时间：
2009-04-01
影响因子：
14.9
通讯作者：
Inoue, Kunio
中科院分区：
文献类型：
--
作者：
Fukumura, Kazuhiro;Taniguchi, Ichiro;Inoue, Kunio
在剪接过程中，U1 snRNP在5个剪接位点的识别中起着至关重要的作用。在这里，我们报告了人类中自然发生的不依赖于u1的u2型剪接的第一个例子。在体外实验中，在人F1 (hF1) 9内含子上形成的剪接前E复合体中不包含U1成分。此外，即使在被u1破坏的爪蟾卵母细胞和被u1灭活的HeLa核提取物中，hF1内含子9也能有效地剪接。最后，当内含子9变为依赖于u1的内含子时，由另一种剪接调节因子Fox-1诱导的hF1外显子9跳变受损。我们的研究结果表明，不依赖于u1的剪接有助于调控一类pre- mrna的选择性剪接。
U1 snRNP plays a crucial role in the 5 splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1 (hF1) intron 9 in vitro. Moreover, hF1 intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1 exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.