Effect of ultraviolet photoactivation of titanium on osseointegration in a rat model.
Effect of ultraviolet photoactivation of titanium on osseointegration in a rat model.
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发表时间：
2010-03
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通讯作者：
T. Ueno;M. Yamada;N. Hori;Takeo Suzuki;T. Ogawa
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作者：
T. Ueno;M. Yamada;N. Hori;Takeo Suzuki;T. Ogawa
目的本研究的目的是确定紫外（UV）光处理钛种植体是否可以增强骨整合，以充分克服大鼠股骨模型中较短种植体的负面影响。材料与方法制备长度为2mm（长种植体）和1.2 mm（短种植体）的酸蚀微型钛种植体。其中一些植入物在手术前用紫外线照射48小时。在大鼠模型中使用生物力学植入物推入试验来评估这些植入物产生的骨整合强度。通过扫描电镜观察组织形态和能量色散x射线光谱分析元素组成。结果在愈合第4周和第8周，较长种植体的推入测试值分别比较短种植体高80%和100%。较短种植体的紫外线处理显著增加了它们的推入值，在第2周时，推入值比未治疗的较长种植体高100%，在第4周和第8周时，推入值与未治疗的较长种植体相当。推入测试后的扫描电子显微照片和能量色散x射线光谱检查显示，与未处理的表面相比，紫外线处理的种植体表面被含有钙和磷的骨或组织残留物覆盖的范围更广。紫外处理后，钛表面由疏水性转变为超亲水性，但获得的超亲水性与生物效应之间的因果关系尚不清楚。结论：在本研究范围内，紫外光预处理可显著提高酸蚀钛种植体的骨整合能力。采用微型种植体进行紫外处理，克服了种植体长度缩短40%的骨整合缺陷。这些结果需要在其他更接近人类牙种植体的动物模型和种植体中得到证实，以确定真正的临床意义。
PURPOSE The objective of this study was to determine whether ultraviolet (UV) light treatment of titanium implants could enhance osseointegration to sufficiently overcome the negative aspects of shorter implants in a rat femur model. MATERIALS AND METHODS Acid-etched miniature titanium implants with lengths of 2 mm (longer implants) and 1.2 mm (shorter implants) were prepared. Some of these implants were treated with UV light for 48 hours prior to surgery. The strength of osseointegration generated by these implants was evaluated using a biomechanical implant push-in test in a rat model. Peri-implant osteogenesis was examined by scanning electron microscopy for tissue morphology and energy dispersive x-ray spectroscopy for elemental composition. RESULTS Push-in test values for the longer implants were 80% and 100% greater than those of the shorter implants at weeks 4 and 8 of healing, respectively. UV treatment of the shorter implants significantly increased their push-in value, resulting in a 100% higher value than untreated longer implants at week 2 and a push-in value that was equivalent to that of the untreated longer implants at weeks 4 and 8. Scanning electron micrographs and energy dispersive x-ray spectroscopic examinations after push-in testing revealed that the UV-treated implant surfaces were covered more extensively by bone or tissue remnants containing calcium and phosphorous than the untreated surfaces. The titanium surfaces were converted from hydrophobic to superhydrophilic status after UV treatment, although the cause-result relationship between the acquired superhydrophilicity and biologic effects remained unclear. CONCLUSIONS Within the limits of this investigation, UV light pretreatment substantially enhanced the osseointegration capacity of acid-etched titanium implants. The deficiencies of osseointegration in implants with a 40% shorter length were overcome by UV treatment in the rat model using miniature implants. These results need to be confirmed in other animal models and implants that more closely resemble human dental implants to determine the true clinical significance.