Genes with increased transcript levels following harvest of the sporophore of Agaricus bisporus have multiple physiological roles
Genes with increased transcript levels following harvest of the sporophore of Agaricus bisporus have multiple physiological roles
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DOI：
10.1016/s0953-7562(08)61993-0
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发表时间：
2001-10-01
影响因子：
--
通讯作者：
Burton, KS
中科院分区：
文献类型：
--
作者：
Eastwood, DC;Kingsnorth, CS;Burton, KS
我们从双孢菇收获和储存的孢子叶中筛选出一个cDNA文库，鉴定出19个转录水平高于收获时的基因。这些基因中有五个在从菌丝体上分离之前没有检测到mRNA水平。对10个克隆的序列分析表明，这些基因与已知基因有显著的相似性，这些基因编码的蛋白质涉及聚合物分解和代谢、细胞壁合成、逆境耐受性、细胞色素P450活性和DNA结合。这些基因功能的多样性表明，采收后孢子体的变化涉及几个不同的生理过程。
We screened a cDNA library generated from harvested and stored sporophores of Agaricus bisporus and identified 19 genes with higher transcript levels than at the time of harvest. Five of these genes had no detectable mRNA levels prior to detachment from the mycelium. Sequence analysis of ten clones revealed significant similarities to known genes, these code for proteins involved in polymer breakdown and metabolism, cell wall synthesis, stress tolerance, cytochrome P450 activity and DNA binding. The diversity of functions of these genes suggests the changes in the sporophore after harvest involve several different physiological processes.