Steps towards a repertoire of comprehensive maps of human protein interaction networks: the Human Proteotheque Initiative (HuPI)
Steps towards a repertoire of comprehensive maps of human protein interaction networks: the Human Proteotheque Initiative (HuPI)
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DOI：
10.1139/o08-006
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发表时间：
2008-04-01
影响因子：
2.9
通讯作者：
Jeronimo, Celia
中科院分区：
文献类型：
--
作者：
Coulombe, Benoit;Blanchette, Mathieu;Jeronimo, Celia
定义人类蛋白质相互作用网络对于全面、基于系统地理解在正常和疾病条件下维持细胞生长的分子事件已经变得至关重要。为了表征来自人类细胞的蛋白质相互作用网络，我们承担了一项系统化、不偏不倚的技术管道的开发，该管道结合了实验和计算方法。这个发现引擎是人类蛋白质组倡议(HuPI)的核心，这是一个多学科项目，旨在建立人类蛋白质相互作用网络的全面图谱，即人类蛋白质组。Proteotheque中包含的信息通过一个交互式网站公开提供，可以参考该网站来可视化人类细胞中形成的一些基本分子连接，并通过关联来确定以前未表征的蛋白质的假定功能。描述了HuPI向成为准确和完整的蛋白质相互作用图库发展的过程。
Defining human protein interaction networks has become essential to develop an overall, systems-based understanding of the molecular events that sustain cell growth in normal and disease conditions. To characterize protein interaction networks from human cells, we have undertaken the development of a systematic, unbiased technology pipeline that couples experimental and computational approaches. This discovery engine is central to the Human Proteotheque Initiative (HuPI), a multidisciplinary project aimed at building a repertoire of comprehensive maps of human protein interaction networks, the Human Proteotheque. The information contained in the Proteotheque is made publicly available through an interactive web site that can be consulted to visualize some of the fundamental molecular connections formed in human cells and to determine putative functions of previously uncharacterized proteins based on guilt by association. The process governing the evolution of HuPI towards becoming a repository of accurate and complete protein interaction maps is described.