A NEW GENE, BCM, ON CHROMOSOME-16 IS FUSED TO THE INTERLEUKIN-2 GENE BY A T(4-16)(Q26-P13) TRANSLOCATION IN A MALIGNANT T-CELL LYMPHOMA
A NEW GENE, BCM, ON CHROMOSOME-16 IS FUSED TO THE INTERLEUKIN-2 GENE BY A T(4-16)(Q26-P13) TRANSLOCATION IN A MALIGNANT T-CELL LYMPHOMA
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DOI：
10.1002/j.1460-2075.1992.tb05482.x
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发表时间：
1992-11-01
期刊：
影响因子：
11.4
通讯作者：
TSAPIS, A
中科院分区：
文献类型：
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作者：
LAABI, Y;GRAS, MP;TSAPIS, A
A t（4; 16）（q26; p13.1）染色体易位，将通常位于染色体带4 q26上的白细胞介素2基因与来自染色体带16p13.1的序列重新排列。用白细胞介素2基因特异性探针筛选肿瘤细胞的cDNA文库。分离出三个克隆，其从5'到3'由白细胞介素2基因的三个第一外显子和编码181个氨基酸的16 p13读框内序列组成。衍生自该序列的探针在显示成熟B淋巴细胞特征的各种细胞系中检测到1.2 kb转录物，但在代表其他造血谱系的其他细胞系或其他器官中未检测到。由于这个原因，这个新基因被命名为B细胞成熟调控基因。正常人cDNA的开放阅读框架预测了一个184个氨基酸的蛋白质，具有一个单一的跨膜结构域，与数据库中存储的任何蛋白质序列都没有同源性。我们的数据表明，CD 45是一个新的基因，其表达与B细胞终末成熟相一致。
A t(4;16)(q26;p13.1) chromosome translocation found in tumour cells from a patient with a T cell lymphoma was shown to rearrange the interleukin 2 gene, normally located on chromosome band 4q26, with sequences from chromosome band 16p13.1. A cDNA library of tumour cells was screened with an interleukin 2 gene-specific probe. Three clones were isolated, which consisted, from 5' to 3', of the three first exons of the interleukin 2 gene followed by a 16p13 in-frame sequence encoding 181 amino acids. A probe derived from this sequence detected a 1.2 kb transcript in various cell lines exhibiting mature B lymphoid cell features, but this was not detected in other cell lines representative of other haematopoietic lineages, or in other organs. For this reason, the novel gene was termed BCM for B cell maturation. The open reading frame of BCM normal cDNA predicted a 184 amino acid protein with a single transmembrane domain which had no homology with any protein sequence stored in data banks. Our data indicate that BCM is a new gene whose expression coincides with B cell terminal maturation.