ANALYSIS OF THE OTSBA OPERON FOR OSMOREGULATORY TREHALOSE SYNTHESIS IN ESCHERICHIA-COLI AND HOMOLOGY OF THE OTSA AND OTSB PROTEINS TO THE YEAST TREHALOSE-6-PHOSPHATE SYNTHASE PHOSPHATASE COMPLEX
ANALYSIS OF THE OTSBA OPERON FOR OSMOREGULATORY TREHALOSE SYNTHESIS IN ESCHERICHIA-COLI AND HOMOLOGY OF THE OTSA AND OTSB PROTEINS TO THE YEAST TREHALOSE-6-PHOSPHATE SYNTHASE PHOSPHATASE COMPLEX
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DOI：
10.1016/0378-1119(94)90316-6
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发表时间：
1994-07-22
期刊：
影响因子：
3.5
通讯作者：
STROM, AR
中科院分区：
文献类型：
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作者：
KAASEN, I;MCDOUGALL, J;STROM, AR
对位于min 42的大肠杆菌otsBA操纵子进行测序，发现其编码一个29.1 kda的海藻糖-6-磷酸磷酸酶（OtsB）和一个53.6 kda的海藻糖-6-磷酸合成酶（OtsA）。这两种蛋白均与由TPS1、TPS2和TPS3 （TSL1）亚基组成的酵母海藻糖-6-磷酸合成酶/磷酸酶复合体的亚基序列同源。OtsA与全长TPS1、TPS2的n端部分以及TPS3的一个内部区域（TSL1）具有同源性。OtsB与TPS2的c端有同源性，但与其他亚基无同源性。引物延伸分析显示，无论生长条件如何，在otsB上游和otsA上游各有一个转录起点。通过蛋白n端序列测定，确定了otsB和otsA基因的起始密码子。otsB编码区的3‘端与otsA编码区的5’端重叠23个核苷酸。araH基因位于otsBA的上游，otsB可能与pexA相同。
The Escherichia coli otsBA operon, located at min 42, was sequenced and shown to encode a 29.1-kDa trehalose-6-phosphate phosphatase (OtsB) and a 53.6-kDa trehalose-6-phosphate synthase (OtsA). Both proteins display sequence homology with subunits of the Saccharomyces cerevisiae trehalose-6-phosphate synthase/phosphatase complex, which is made up of the subunits TPS1, TPS2 and TPS3 (TSL1). OtsA has homology to the full-length TPS1, the N-terminal part of TPS2 and an internal region of TPS3 (TSL1). OtsB has homology to the C-terminal part of TPS2, but no homology to the other subunits. Primer extension analysis showed only one transcription start point upstream from otsB and one upstream from otsA, regardless of the growth conditions tested. The start codons of the otsB and otsA genes were established by N-terminal sequence determination of the proteins. The 3' end of the otsB coding region overlaps the 5' end of the otsA coding region by 23 nucleotides. The araH gene is located directly upstream from otsBA, and otsB may be identical to pexA.