Planar Airy beam light-sheet for two-photon microscopy.
Planar Airy beam light-sheet for two-photon microscopy.
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DOI：
10.1364/boe.395547
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发表时间：
2020-07-01
影响因子：
3.4
通讯作者：
Vettenburg T
中科院分区：
文献类型：
--
作者：
Hosny NA;Seyforth JA;Spickermann G;Mitchell TJ;Almada P;Chesters R;Mitchell SJ;Chennell G;Vernon AC;Cho K;Srivastava DP;Forster R;Vettenburg T
我们展示了第一个平面艾里光片显微镜。荧光光片显微镜已成为研究具有细胞或亚细胞分辨率的大型生物样品的首选方法。传播不变性艾里光束使视场在单光子激发下增加十倍；然而，光片的非对称性限制了其多光子激发的潜力。在这里，我们展示了如何从弯曲的传播不变艾里光束形成平面光片。由此产生的对称光片在扩展的视场内均匀地激发双光子荧光，而不需要反褶积。我们展示了快速双光子成像的方法大体积的神经元组织。
We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.