Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
复制标题
DOI：
10.3791/62468
复制
发表时间：
2021-05-01
影响因子：
1.2
通讯作者：
Hayashi, Hisaysohi
中科院分区：
文献类型：
--
作者：
Hempstock, Wendy;Ishizuka, Noriko;Hayashi, Hisaysohi
1951年，丹麦科学家Hans Ussing首次发明了Ussing室技术，用于研究钠在青蛙皮肤中的跨细胞转运。从那时起，这项技术已被应用于许多不同的组织，以研究跨膜运输的生理参数。尤辛室方法优于其他方法，因为可以使用天然组织，使其更适用于体内发生的情况。然而，因为使用天然组织，所以通量低，时间有限，并且组织制备需要技能和培训。这些腔室已被用于研究各种组织中的特定转运蛋白，了解疾病病理生理学，如囊性纤维化，研究药物转运和摄取，特别是有助于了解肠道中的营养物质转运。鉴于组织的整个上皮转运过程，不仅跨上皮途径，而且细胞旁途径也很重要。紧密连接是跨肠的组织特异性细胞旁渗透性的关键决定因素。在这篇文章中，Ussing室技术将被用来评估旁细胞的离子透过选择性，通过测量跨上皮电导和稀释电位。
The Ussing chamber technique was first invented by the Danish scientist Hans Ussing in 1951 to study the transcellular transport of sodium across frog skin. Since then, this technique has been applied to many different tissues to study the physiological parameters of transport across membranes. The Ussing chamber method is preferable to other methods because native tissue can be used, making it more applicable to what is happening in vivo. However, because native tissue is used, throughput is low, time is limited, and tissue preparation requires skill and training. These chambers have been used to study specific transporter proteins in various tissues, understand disease pathophysiology such as in Cystic Fibrosis, study drug transport and uptake, and especially contributed to the understanding of nutrient transport in the intestine. Given the whole epithelial transport process of a tissue, not only transepithelial pathways, but also paracellular pathways are important. Tight junctions are a key determinant of tissue specific paracellular permeability across the intestine. In this article, the Ussing chamber technique will be used to assess paracellular permselectivity of ions by measuring transepithelial conductance and dilution potentials.