Skeletal muscle metabolism in individuals with spinal cord injury
Skeletal muscle metabolism in individuals with spinal cord injury
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DOI：
10.1152/japplphysiol.00094.2011
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发表时间：
2011-07-01
影响因子：
3.3
通讯作者：
Zhao, Qun
中科院分区：
文献类型：
--
作者：
McCully, Kevin K.;Mulcahy, Tara K.;Zhao, Qun
McCully KK，Mulcahy TK，Ryan TE，Zhao Q.脊髓损伤患者的骨骼肌代谢。J Appl Physiol 111：143-148，2011.首次发表于2011年4月21日; doi：10.1152/japplphysiol.00094.2011。随着脊髓损伤（SCI）患者存活率的提高，代谢和心血管疾病的检测和预防变得越来越重要。很少有研究评估瘫痪骨骼肌中线粒体的体内功能。本研究的目的是比较氧化肌肉代谢使用率的磷酸肌酸（PCr）再合成测量的磁共振波谱（MRS）在人与脊髓损伤和健全（AB）的控制。8例完全SCI受试者（美国脊髓损伤协会损伤量表A，T3-T12级，损伤持续时间2-13年）与12例AB对照进行了比较。采用T1加权（1）H MR扫描大腿以识别骨骼肌。在3 T临床MRI扫描仪中，将13 x 13 cm（2）表面线圈放置在右股外侧肌上进行磷MRS。PCr再合成后测定60秒电刺激后，在4 Hz的SCI和AB和AB科目后39秒的自愿等长收缩。除了磷酸二酯峰升高外，SCI和AB之间的静息代谢物没有差异。与自愿运动相比，使用电刺激的AB受试者的PCr恢复较慢（28.4 +/- 6.1 vs. 41.5 +/- 4.3 s; P < 0.05）。脊髓损伤组PCr恢复率和计算的肌肉最大氧化能力均为电刺激AB组的52%（P < 0.001）。瘫痪肌肉的体内氧化代谢降低程度与线粒体肌病和心力衰竭患者相似。
McCully KK, Mulcahy TK, Ryan TE, Zhao Q. Skeletal muscle metabolism in individuals with spinal cord injury. J Appl Physiol 111: 143-148, 2011. First published April 21, 2011; doi:10.1152/japplphysiol.00094.2011.-With increasing survival rates in people with spinal cord injuries (SCI), detection and prevention of metabolic and cardiovascular disease have become increasingly important. Few studies have evaluated in vivo mitochondrial function in paralyzed skeletal muscle. The purpose of this study was to compare oxidative muscle metabolism using the rate of phosphocreatine (PCr) resynthesis measured by magnetic resonance spectroscopy (MRS) in people with SCI and able-bodied (AB) controls. Eight subjects with complete SCI (American Spinal Injury Association Impairment Scale A, levels T3-T12, injury duration 2-13 years) were compared with 12 AB controls. T1-weighted (1)H MR images of the thigh were taken to identify skeletal muscle. Phosphorous MRS was performed with a 13 x 13-cm(2) surface coil placed on the right vastus lateralis in a 3 Tesla clinical MRI scanner. PCr resynthesis was measured after electrical stimulation for 60 s at 4 Hz in SCI and AB and in AB subjects after 39 s of voluntary isometric contractions. Resting metabolites were not different between SCI and AB, except for an elevated phosphodiester peak. PCr recovery was slower in AB subjects using electrical stimulation compared with voluntary exercise (28.4 +/- 6.1 vs. 41.5 +/- 4.3 s; P < 0.05). PCr recovery rates and calculated muscle maximum oxidative capacity in SCI were both 52% of electrically stimulated AB (P < 0.001). In vivo oxidative metabolism was reduced in paralyzed muscle to a similar extent as seen in people with mitochondrial myopathies and heart failure.