On the very rapid enhancement by D2O of the temperature-tolerance of adult Drosophila.
On the very rapid enhancement by D2O of the temperature-tolerance of adult Drosophila.
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关于 D2O 快速增强成年果蝇的温度耐受性。
DOI：
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发表时间：
1974
影响因子：
11.1
通讯作者：
E. Cosbey
中科院分区：
文献类型：
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作者：
C. Pittendrigh;E. Cosbey
氧化氘（D（2）O）以蔗糖溶液的形式给予成年果蝇时，可增加拟暗果蝇的温度耐受性。这种效果非常迅速地发挥出来;在苍蝇有短暂的机会（10分钟）饮水后10分钟内就能检测到。这种对热死亡的抵抗力的增加肯定意味着大分子对热变性的抵抗力的增加。已知D（2）O对体外蛋白质溶液有这种作用。增加的稳定性发展的速度清楚地暗示了D（2）O的溶剂作用，并且如果该作用可追溯到有助于构象稳定性的疏水相互作用的增强，则最容易理解。然而，不能排除氘代氢键强度的增加。简要地考虑了这些结果与D（2）O总是通过“降低表观温度”来增加昼夜振荡周期的假设的可能关系。
Deuterium oxide, D(2)O, increases the temperature-tolerance of Drosophila pseudoobscura when it is administered to adult flies as a sucrose solution. The effect is very rapidly exerted; it is detected within 10 min after the flies have a brief (10 min) opportunity to drink. This increased resistance to heat-death surely implies an increased resistance of macromolecules to thermal denaturation. D(2)O is known to exert such an effect on in vitro protein solutions. The speed with which the increased stability develops clearly implicates a solvent action of D(2)O, and is most easily understood if the effect is traceable to enhancement of the hydrophobic interactions which contribute to conformational stability. The increased strength of deuterated hydrogen bonds cannot be excluded however. The possible bearing of these results on the hypothesis that D(2)O always increases the period of circadian oscillations by "diminishing the apparent temperature" is briefly considered.