Up-regulated expression of Bnip3L after intracerebral hemorrhage in adult rats
Up-regulated expression of Bnip3L after intracerebral hemorrhage in adult rats
复制标题
成年大鼠脑出血后Bnip3L表达上调
DOI：
10.1007/s10735-013-9506-7
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发表时间：
2013-10-01
影响因子：
3.2
通讯作者：
Cao, Maohong
中科院分区：
文献类型：
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作者：
Rui, Ying;Ke, Kaifu;Cao, Maohong
Bnip3L，也称为 NIX，是 E1B 19K/Bcl-2 结合和促凋亡蛋白 Bnip3 的同源物，它可以与 Bcl-2 结合以发挥这种作用。在肿瘤细胞中，Bnip3L 在抑制肿瘤生长中发挥作用，但一些研究认为，通过 Bnip3L 缺氧诱导的自噬是一种促进肿瘤进展的生存机制。在心肌中，它与心肌功能下降有关。然而，其在脑出血（ICH）中的作用仍不清楚。在该框架中，我们发现成年大鼠在进行 ICH 后血肿周围区域的 Bnip3L 表达增加。双重免疫荧光染色表明 Bnip3L 与神经元共存，而不是星形胶质细胞或少突胶质细胞。此外，我们检测到神经元凋亡标志物活性 caspase-3 与 Bnip3L 存在共定位。此外，还发现了 Bnip3L 和 Bcl-2 之间的共定位和共免疫沉淀，与之前的研究一致。我们所有的研究结果表明 Bnip3L 可能参与 ICH 的病理生理学。
Bnip3L, also known as NIX, is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3 which can bind to Bcl-2 to elaborate that effect. In tumor cells, Bnip3L played a role in tumor growth inhibition, but some studies argued hypoxia-induced autophagy via Bnip3L was a survival mechanism that promoted tumor progression. In heart muscle, it related to decreased myocardial function. However, its function in intracerebral hemorrhage (ICH) is still not clear. In this frame, we found the Bnip3L expression increased in the perihematomal region in adult rats after performed ICH. Double immunofluorenscence staining manifested that Bnip3L co-located with neurons, not astrocytes or oligodendrocytes. Furthermore, we detected that neuronal apoptosis marker active caspase-3 had colocalizations with Bnip3L. In addition, colocalizations and co-immunoprecipitation between Bnip3L and Bcl-2, consistent with previous study, were also found. All our findings suggested that Bnip3L might be involved in the pathophysiology of ICH.