Recombinant human glial growth factor 2 (rhGGF 2) improves functional recovery of crushed peripheral nerve (a double-blind study)
Recombinant human glial growth factor 2 (rhGGF 2) improves functional recovery of crushed peripheral nerve (a double-blind study)
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DOI：
10.1016/s0197-0186(98)00037-0
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发表时间：
1998-10-01
影响因子：
4.2
通讯作者：
Urbaniak, JR
中科院分区：
文献类型：
--
作者：
Chen, LE;Liu, K;Urbaniak, JR
这项体内双盲研究评估了重组人胶质生长因子 2 (rhGGF2)（一种雪旺细胞有丝分裂原）对挤压损伤后大鼠坐骨神经运动功能恢复的影响。七十三只大鼠被分成三组。 I组（n = 5），假手术； II 组（n = 34）和 III 组（n = 34）在坐骨神经的 5 mm 段上接受 100 g 挤压负载 2 小时。第III组在神经挤压前一天通过皮下注射用1mg/kg rhGGF2治疗，并在接下来的四天内每天注射。第二组接受等量的盐水作为对照。通过计算坐骨功能指数（SFI）和趾长伸肌（EDL）的强直收缩力恢复率来评估运动功能恢复情况。 rhGGF2处理的动物在挤压后第二天神经功能明显恢复，而对照组的神经仍然麻痹。与对照组相比，rhGGF2 治疗的动物在术后第 11-21 天期间显示出 SFI 显着改善。 rhGGF2治疗组的等长性强直收缩力强于对照组，第4天40至70Hz刺激频率下差异显着。相关分析显示，强直性收缩力与SFI呈线性相关。组织学评估表明，与对照组相比，rhGGF2处理的动物表现出较轻的退化和较早的强健轴突髓鞘再生。结果表明，从功能恢复测量和神经形态定性评估中可以看出，rhGGF2 治疗可有效促进神经再生。 GGF 的保护作用机制可能与其直接作用于雪旺细胞、刺激其有丝分裂以及诱导神经元维持和修复所必需的神经营养因子有关。 (C) 1998 Elsevier Science Ltd. 保留所有权利。
This in vivo double-blind study evaluated the effect of recombinant human glial growth factor 2 (rhGGF2), a Schwann cell mitogen, on the recovery of motor function of rat sciatic nerve following crush injury. Seventy three rats were divided into three groups. Group I(n = 5), sham operated; Groups II(n = 34) and III (n = 34) received a 100 g crush load for 2 h over a 5 mm segment of the sciatic nerve. Group III was treated with I mg/kg rhGGF2, via subcutaneous injection one day before nerve crush and daily for the following four days. Group II received an equivalent volume of saline as a control. Motor functional recovery was assessed by calculating the sciatic functional index (SFI) and the recovery rate of tetanic contractile force of the extensor digitorum longus (EDL) muscle. Recovery of nerve function was evident at day II after crush in the rhGGF2-treated animals, whereas the nerves in controls were still paralyzed. The rhGGF2-treated animals showed a significant improvement of the SFI between days 11-21 postoperatively when compared to controls. The isometric tetanic contractile force was stronger in the rhGGF2-treated group than in controls, with a significant difference at 40 to 70 Hz stimulus frequencies on day 4. Correlation analysis showed that tetanic contractile force had a linear correlation with the SFI. Histologic assessment indicated that the rhGGF2-treated animals showed less severe degeneration and earlier robust remyelination of axons than controls. The results suggest that treatment with rhGGF2 is effective in promoting nerve regeneration as seen in measurements of functional recovery and qualitative assessment of nerve morphology. The mechanism of GGF's protective effect may be related to its direct action on Schwann cells, stimulating their mitosis as well as inducing neurotrophic factors essential to neuronal maintenance and repair. (C) 1998 Elsevier Science Ltd. All rights reserved.