Induction of a growth‐phase‐dependent promoter triggers transcription of bolA, an Escherichia coli morphogene.
Induction of a growth‐phase‐dependent promoter triggers transcription of bolA, an Escherichia coli morphogene.
复制标题
生长阶段依赖性启动子的诱导会触发 bolA（一种大肠杆菌形态基因）的转录。
作者：
M. Aldea;T. Garrido;C. Hernández‐Chico;Miguel Vicente;S. R. Kushner
对参与大肠杆菌形态发生途径的bolA基因进行了测序，并鉴定出两个潜在的启动子。bolA结构基因近端启动子P1的表达在生长平稳期的过渡过程中被特异性诱导。该启动子包含一个不寻常的‐‐10区域（CGGCTAGTA），它定义了一类新的大肠杆菌启动子，在对数生长停止期间，大量蛋白质的合成速度急剧增加。这一结论通过鉴定另外两个大肠杆菌启动子和一个质粒启动子得到了证实，这两个启动子也是在过渡到生长固定阶段时被诱导的。对bolA零突变体生长指数期和固定期产生的蛋白质的分析表明，bolA蛋白可能在诱导青霉素结合蛋白6 （PBP6）在过渡到固定期的表达中起作用。支持这一假设的是在bolA编码序列中存在假定的DNA结合结构域。
The bolA gene, which is involved in the morphogenetic pathways of Escherichia coli, was sequenced and two potential promoters were identified. Expression from promoter P1, proximal to the bolA structural gene is specifically induced during the transition to the stationary phase of growth. This promoter contains an unusual‐‐10 region (CGGCTAGTA), which defines a new class of E. coli promoters necessary for the dramatic increase in the rate of synthesis of a large set of proteins during the cessation of logarithmic growth. This conclusion was confirmed by identifying two additional E. coli promoters and one plasmid promoter, which also were induced during the transition to the stationary phase of growth. Analysis of proteins produced during the exponential and stationary phases of growth in a bolA null mutant suggest a possible role for the BolA protein in the induction of the expression of penicillin‐binding protein 6 (PBP6) in the transition to the stationary phase. Supporting this hypothesis is the presence of a putative DNA‐binding domain within the bolA coding sequence.