Cell migration along the lateral cortical stream to the developing basal telencephalic limbic system
Cell migration along the lateral cortical stream to the developing basal telencephalic limbic system
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DOI：
10.1523/jneurosci.3092-06.2006
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发表时间：
2006-11-08
影响因子：
5.3
通讯作者：
Corbin, Joshua G.
中科院分区：
文献类型：
--
作者：
Carney, Rosalind S. E.;Alfonso, Teresa B.;Corbin, Joshua G.
在胚胎发育过程中，侧皮质流（LCS）出现在皮质纹状体边界（CSB），发育中的大脑皮质和纹状体之间的边界。LCS由苍白球和苍白球下衍生的神经祖细胞的混合物组成，这些神经祖细胞迁移到基底端脑的发育结构，最明显的是梨状皮质和杏仁核。使用体外和体内的方法相结合，我们分析了时间，组成，迁移模式，起源和要求的同源结构域含有转录因子GSH 2（基因组筛选的同源框2）在这个突出的迁移流的发展。我们发现，Pax 6（配对盒基因6）阳性pallal-derived和DLX 2（远端少同源框2）阳性subpallal-derived LCS细胞亚群产生在不同的时间窗口在胚胎发生。此外，我们的数据表明，CSB边界不仅是由单独的群体的苍白球和subpallial衍生的祖细胞，有助于LCS，但也是一个亚群的细胞共表达Pax 6和Dlx 2。此外，尽管迁移沿着路线概述了放射状胶质细胞的级联，Dlx 2阳性人口似乎主要迁移在一个明显的链状的方式，LCS迁移细胞被本地产生的CSB与其他的苍白球下结构，如内侧，外侧，或尾神经节隆起的贡献很小。我们进一步证明，LCS的产生依赖于含有同源结构域的基因GSH 2，揭示了端脑发育对GSH 2的新要求。
During embryogenesis, the lateral cortical stream (LCS) emerges from the corticostriatal border (CSB), the boundary between the developing cerebral cortex and striatum. The LCS is comprised of a mix of pallial- and subpallial-derived neural progenitor cells that migrate to the developing structures of the basal telencephalon, most notably the piriform cortex and amygdala. Using a combination of in vitro and in vivo approaches, we analyzed the timing, composition, migratory modes, origin, and requirement of the homeodomain-containing transcription factor Gsh2 ( genomic screened homeobox 2) in the development of this prominent migratory stream. We reveal that Pax6 ( paired box gene 6)-positive pallial-derived and Dlx2 (distal-less homeobox 2)- positive subpallial-derived subpopulations of LCS cells are generated in distinct temporal windows during embryogenesis. Furthermore, our data indicate the CSB border not only is comprised of separate populations of pallial- and subpallial-derived progenitors that contribute to the LCS but also a subpopulation of cells coexpressing Pax6 and Dlx2. Moreover, despite migrating along a route outlined by a cascade of radial glia, the Dlx2-positive population appears to migrate primarily in an apparent chain-like manner, with LCS migratory cells being generated locally at the CSB with little contribution from other subpallial structures such as the medial, lateral, or caudal ganglionic eminences. We further demonstrate that the generation of the LCS is dependent on the homeodomain-containing gene Gsh2, revealing a novel requirement for Gsh2 in telencephalic development.