Enzymatic catalyzed palm oil hydrolysis under ultrasound irradiation: Diacylglycerol synthesis
Enzymatic catalyzed palm oil hydrolysis under ultrasound irradiation: Diacylglycerol synthesis
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DOI：
10.1016/j.ultsonch.2012.11.017
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发表时间：
2013-07-01
影响因子：
8.4
通讯作者：
da Silva, Edson A.
中科院分区：
文献类型：
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作者：
Awadallak, Jamal A.;Voll, Fernando;da Silva, Edson A.
富含二酰基甘油（DAG）的油具有与常规食用油相似的感官特性，但这些油不倾向于积累为脂肪。棕榈油因其成本低廉，在食用油生产方面居世界首位。本研究的目的是提出一种新的方法，通过使用Lipozyme RM IM商业脂肪酶作为催化剂在超声辐射下水解棕榈油生产甘油二酯。反应在55 ℃下用两种不同的方法进行。首先，反应体系在整个反应时间内暴露于超声波，这导致酶失活和水分蒸发。然后在水解反应之前使用超声波促进水/油体系的乳化，避免探针和酶之间的接触。使用实验设计来优化超声相关参数并使水解速率最大化，并且在这些条件下，随着平衡的变化，评估DAG的生产。（水+油质量）水含量，1.36重量%（水+油质量）酶负荷，12小时反应时间，1.2分钟和200 W超声暴露。在这些条件下，甘油二酯产率为34.17重量%。(C)2013年由Elsevier B. V.出版
Diacylglycerol (DAG) rich oils have an organoleptic property like that of regular edible oils, but these oils do not tend to be accumulated as fat. Palm oil ranks first in the world in terms of edible oil production owing to its low cost. The aim of this study was to propose a new methodology to produce diacylglycerol by hydrolysis of palm oil using Lipozyme RM IM commercial lipase as a catalyst under ultrasound irradiation. The reactions were carried out at 55 degrees C with two different methods. First, the reaction system was exposed to ultrasonic waves for the whole reaction time, which led to enzymatic inactivation and water evaporation. Ultrasound was then used to promote emulsification of the water/oil system before the hydrolysis reaction, avoiding contact between the probe and the enzymes. An experimental design was used to optimize the ultrasound-related parameters and maximize the hydrolysis rate, and in these conditions, with a change in equilibrium, DAG production was evaluated.Better reaction conditions were achieved for the second method: 11.20 wt.% (water + oil mass) water content, 1.36 wt.% (water + oil mass) enzyme load, 12 h of reaction time, 1.2 min and 200 W of exposure to ultrasound. In these conditions diacylglycerol yield was 34.17 wt.%. (C) 2013 Published by Elsevier B.V.