ATTACHMENT OF TERMINAL N-ACETYLGLUCOSAMINE TO ASPARAGINE-LINKED OLIGOSACCHARIDES OCCURS IN CENTRAL CISTERNAE OF THE GOLGI STACK
ATTACHMENT OF TERMINAL N-ACETYLGLUCOSAMINE TO ASPARAGINE-LINKED OLIGOSACCHARIDES OCCURS IN CENTRAL CISTERNAE OF THE GOLGI STACK
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DOI：
10.1016/0092-8674(85)90161-8
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
ROTHMAN, JE
中科院分区：
文献类型：
--
作者：
DUNPHY, WG;BRANDS, R;ROTHMAN, JE
利用单克隆抗体和电镜技术，将n -乙酰氨基葡萄糖转移酶I定位在高尔基体内。这种酶开始将asn - linoaccharides转化为复合物类型。这种酶集中在高尔基体的中央（或内侧）池中。在高尔基复合体的顺式和反式末端的池肌似乎缺乏这种蛋白质。这些实验确定了高尔基体内侧部分的功能，并暗示高尔基体至少分为3个生化和形态学上不同的贮池室。
Using monoclonal antibodies and EM, N-acetylglucosamine transferase I was localized within the Golgi apparatus. This enzyme initiates the conversion of Asn-lined oligosaccharides to the complex type. The enzyme is concentrated in the central (or medial) cisternae of the Golgi stack. Cisternae at the cis and trans ends of the Golgi complex appear to lack this protein. These experiments establish a function for the medial portion of the Golgi and imply that the Golgi is partitioned into at least 3 biochemically and morphologically distinct cisternal compartments.