Molecular Mapping of Sebaceous Squalene by Ambient Mass Spectrometry
Molecular Mapping of Sebaceous Squalene by Ambient Mass Spectrometry
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DOI：
10.1021/acs.analchem.1c03983
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发表时间：
2021-12-14
影响因子：
7.4
通讯作者：
Shiea, Jentaie
中科院分区：
文献类型：
--
作者：
Cho, Yi-Tzu;Su, Hung;Shiea, Jentaie
角鲨烯(SQ)是一种高度不饱和的皮脂，对人体皮肤起着重要的保护作用。为了更好地了解SQ在临床医学中的作用，需要一种有效的分析方法来全面研究SQ在皮肤不同部位的分布。在这项研究中，用取样探头从一名志愿者的不同表皮区域采集皮脂。然后用热解吸-电喷雾电离/质谱仪(TD-ESI/MS)对探针上的脂类进行表征，每次TD-ESI/MS分析在几秒钟内完成，无需任何样品前处理。表皮角鲨烯在全身皮肤上的分子图谱是通过基于温度颜色梯度缩放SQ的提取离子电流(EIC)的峰面积来绘制的，其中颜色被分配给志愿者3D地图上的1357个采样位置。图像显示，SQ在面部的分布比身体其他任何部位都要高，这表明SQ在保护面部皮肤方面的作用。这一结果与以前使用SQ作为皮脂腺活性标志物的研究是一致的。这项工作的新颖性和意义归结为两点：(1)直接和快速地检测所有主要的皮脂脂数据，包括不饱和碳氢化合物(SQ)和非极性脂类(如胆固醇)。与其他常规和环境电离质谱学方法相比，该结果是独一无二的。(2)这是首次用高通量方法分析全身皮肤上SQ分布的研究。
Squalene (SQ), a highly unsaturated sebaceous lipid, plays an important role in protecting human skin. To better understand the role of SQ in clinical medicine, an efficient analytical approach is needed to comprehensively study the distribution of SQ on different parts of the skin. In this study, sebaceous lipids were collected from different epidermal areas of a volunteer with sampling probes. Thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS) was then used to characterize the lipid species on the probes, and each TD-ESI/MS analysis was completed within a few seconds without any sample pretreatment. The molecular mapping of epidermal squalene on whole-body skin was rendered by scaling the peak area of the extracted ion current (EIC) of SQ based on a temperature color gradient, where colors were assigned to the 1357 sampling locations on a 3D map of the volunteer. The image showed a higher SQ distribution on the face than any other area of the body, indicating the role of SQ in protecting facial skin. The results were in agreement with previous studies using SQ as a marker to explore sebaceous activity. The novelty and significance of this work are concluded as two points: (1) direct and rapid detection of all major dasses of sebaceous lipids, including the unsaturated hydrocarbons (SQ) and nonpolar lipids (e.g., cholesterol). The results are unique compared to other conventional and ambient ionization mass spectrometry methods and (2) this is the first study to analyze SQ distribution on the whole-body skin by a high-throughput approach.