Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures
Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures
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DOI：
10.1128/jcm.38.3.1203-1208.2000
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发表时间：
2000-03-01
影响因子：
9.4
通讯作者：
Montenegro-James, S
中科院分区：
文献类型：
--
作者：
Caviedes, L;Lee, TS;Montenegro-James, S
廉价、快速、可靠的检测结核分支杆菌（MTB）感染和药物敏感性的方法对于控制结核病至关重要。新的显微镜观察肉汤药物敏感性试验（MODS）检测MTB在液体培养基中的早期生长，允许更及时的诊断和药物敏感性测试。从秘鲁住院患者的痰液样本进行了分析，使用染色，文化，和PCR。MODS的敏感性（92%）与最敏感的其他培养方法（93%）相比毫不逊色。结核病阳性痰标本用微孔亚甲蓝法（MABA）和MODS法检测异烟肼和利福平的敏感性。在89%的病例中，MODS和MABA之间存在一致性。在所使用的诊断和药敏试验方法中，MODS产生结果最快（中位数分别为9.0和9.5天）。MODS是一种快速、廉价、敏感和特异的MTB检测和药敏试验方法;特别适用于感染率高和多重耐药病例数量增加的发展中国家。
Inexpensive, rapid, and reliable methods of detecting infection by and drug susceptibility of Mycobacterium tuberculosis (MTB) are crucial to the control of tuberculosis. The novel microscopic observation broth-drug susceptibility assay (MODS) detects early growth of MTB in liquid medium, allowing more timely diagnosis and drug susceptibility testing. Sputum samples from hospitalized patients in Peru were analyzed by using stains, culture, and PCR. Sensitivity of MODS (92%) compared favorably with the most sensitive of the other culture methods (93%). Sputum samples positive for tuberculosis were tested for susceptibility to isoniazid and rifampin with the microwell alamar blue assay (MABA) and MODS. In 89% of cases, there was concordance between MODS and MABA. Of the diagnostic and susceptibility testing methods used, MODS yielded results most rapidly (median, 9.0 and 9.5 days, respectively). MODS is a rapid, inexpensive, sensitive, and specific method for MTB detection and susceptibility testing; it is particularly appropriate for use in developing countries burdened by significant infection rates and increasing numbers of multiple-drug-resistant cases.