Theranostic imaging of liver cancer using targeted optical/MRI dual-modal probes.
Theranostic imaging of liver cancer using targeted optical/MRI dual-modal probes.
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DOI：
10.18632/oncotarget.15642
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发表时间：
2017-05-16
期刊：
影响因子：
--
通讯作者：
Tian J
中科院分区：
文献类型：
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作者：
Chen Q;Shang W;Zeng C;Wang K;Liang X;Chi C;Liang X;Yang J;Fang C;Tian J
影像学技术提供的准确术前检测和术中导航对肝癌手术的成功具有重要影响。然而，使用任何单一模态成像方法在诊断和外科治疗过程中都难以实现令人满意的性能。在这里，我们报告了一种新的双模态磁共振成像（MRI）和近红外荧光（NIRF）探针的合成和特性，并验证其在裸鼠肝癌模型中的可行性。探针由包被有脂质体的超顺磁性氧化铁（SPIO）纳米颗粒组成，脂质体上结合有肿瘤靶向剂、Arg-Gly-Asp肽（RGD）和NIRF染料（吲哚菁绿色，ICG）。检测了MRI-NIRF探针的特异性靶向、生物分布和成像能力。此外，我们应用双模态方法对原位肝肿瘤和肝内肿瘤转移的小鼠模型进行根治性切除术的术前诊断和术中指导。该研究表明，MRI和荧光图像在探针注射后显示出清晰的肿瘤轮廓（SPIO@脂质体-ICG-RGD）。注射后MRI获得的对比噪声比为31.9 ± 25.4，用于术前诊断，这有助于检测小肿瘤（0.9 ± 0.5 mm）。在注射后72 h，NIRF成像的最大肿瘤与背景比为2.5 ± 0.3，可有效捕获术中微小肿瘤病灶（0.6 ± 0.3 mm）。新型MRI-NIRF双模态探头有望实现更准确的肝脏肿瘤检测和切除。
The accurate preoperative detection and intraoperative navigation afforded by imaging techniques have had significant impact on the success of liver cancer surgeries. However, it is difficult to achieve satisfactory performance in both diagnosis and surgical treatment processes using any single modality imaging method. Here, we report the synthesis and characteristics of a novel dual-modality magnetic resonance imaging (MRI) and near-infrared fluorescence (NIRF) probe and verify its feasibility in nude mouse models with liver cancer. The probes are comprised of superparamagnetic iron oxide (SPIO) nanoparticles coated with liposomes to which a tumor-targeted agent, Arg-Gly-Asp peptides (RGD), and a NIRF dye (indocyanine green, ICG) have been conjugated. Specific targeting, biodistribution, and the imaging ability of the probes for MRI-NIRF were examined. Furthermore, we applied the dual-modality methodology toward the preoperative diagnosis and intraoperative guidance of radical resection in mouse models with both orthotopic liver tumors and intrahepatic tumor metastasis. The study demonstrated that both MRI and fluorescent images showed clear tumor delineation after probe injection (SPIO@Liposome-ICG-RGD). The contrast-to-noise ratio obtained from MRI was 31.9 ± 25.4 at post-injection for the preoperative diagnosis, which is helpful for detecting small tumors (0.9 ± 0.5 mm). The maximum tumor to background ratio of NIRF imaging was 2.5 ± 0.3 at 72 h post-injection for effectively capturing miniscule tumor lesions (0.6 ± 0.3 mm) intraoperatively. The novel MRI-NIRF dual modality probes are promising for the achievement of more accurate liver tumor detection and resection.