Detection of IgG antibodies specific for measles virus by enzyme-linked immunosorbent assay (ELISA).
Detection of IgG antibodies specific for measles virus by enzyme-linked immunosorbent assay (ELISA).
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通过酶联免疫吸附测定 (ELISA) 检测麻疹病毒特异性 IgG 抗体。
作者：
S. Kahane;V. Goldstein;I. Sarov
描述了一种固相酶联免疫吸附测定法（ELISA），用于测定麻疹病毒IgG抗体。该试验使用抗原包被的聚苯乙烯微孔板。该抗原由麻疹感染的Vero细胞的超声提取物组成。用山羊和抗人IgG-过氧化物酶偶联物检测与病毒抗原结合的人IgG。对63名健康成人、11名幼儿和36名患者的血清进行了麻疹病毒IgG滴度检测。将ELISA法与血凝抑制（HI）法或补体固定法的结果进行了比较，结果表明两种方法具有良好的一致性。健康成人的几何平均滴度（GMT） ELISA为753，HI为32.8。如果将这些平均值作为比较的衡量标准，那么ELISA的敏感性大约是HI的23倍。ELISA技术操作迅速，可推荐用于常规诊断。
A solid-phase, enzyme-linked immunosorbent assay (ELISA) for determination of IgG antibodies against measles virus is described. The assay utilized antigen-coated polystyrene microplates. The antigen consisted of a sonicated extract of measles-infected Vero cells. Goat and anti-human IgG-peroxidase conjugate was used to detect human IgG bound to viral antigen. Sera taken from 63 healthy adults, 11 young children and 36 patients were evaluated for their IgG titer against measles virus. Comparison of results obtained by ELISA with those obtained by hemagglutination-inhibition (HI) assay or by complement fixation showed good agreement between the tests. The geometric mean titer (GMT) for healthy adults was 753 for ELISA and 32.8 for HI. If these averages are taken as a measure of comparison, then ELISA is approximately 23 times more sensitive than HI. ELISA technique is rapid to perform and could be recommended for routine diagnosis.