Comprehensive analysis of differentially expressed profiles of Alzheimer's disease associated circular RNAs in an Alzheimer's disease mouse model
Comprehensive analysis of differentially expressed profiles of Alzheimer's disease associated circular RNAs in an Alzheimer's disease mouse model
复制标题
阿尔茨海默病小鼠模型中阿尔茨海默病相关环状RNA差异表达谱的综合分析
DOI：
10.18632/aging.101387
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发表时间：
2018-02-01
期刊：
影响因子：
5.2
通讯作者：
Zhong, Zhen-Guo
中科院分区：
文献类型：
--
作者：
Huang, Jin-Lan;Qin, Mei-Chun;Zhong, Zhen-Guo
环状RNA（circRNA）是一种新型非编码RNA，因其参与阿尔茨海默病（AD）的发病机制而受到越来越多的关注；然而，很少有研究报道 AD 相关 circRNA 的特征和功能。这里使用circRNA微阵列鉴定了5个月大和10个月大的SAMP8小鼠中的circRNA表达谱，发现与对照小鼠相比，在10个月大的SAMP8小鼠中观察到85个失调的circRNA，并且在10个月大的SAMP8与5个月大的SAMP8小鼠中，231个circRNA表现出差异表达。选择一种最显着失调的 circRNA mmu_circRNA_017963 进行基因肿瘤学 (GO) 和通路分析。结果表明，mmu_circRNA_017963与自噬体组装、胞吐作用、凋亡过程、运输和RNA剪接密切相关，并且与囊泡运输途径中的突触小泡周期、剪接体、糖胺聚糖和SNARE相互作用高度相关。总的来说，这项研究首次描述了SAMP8不同年龄的circRNA表达，将有助于理解circRNA在AD发病机制中的调节作用，并为AD的诊断和治疗提供宝贵的资源。
Circular RNAs (circRNAs), a novel kind of non-coding RNA, have received increasing attention for their involvement in pathogenesis of Alzheimer's disease (AD); however, few studies have reported in the characterization and function of AD associated circRNAs. Here the expression profiles of circRNAs in 5-and 10-month-old SAMP8 mice were identified using circRNA microarray and found that 85 dysregulated circRNAs were observed in 10-month-old SAMP8 versus control mice and 231 circRNAs exhibited differential expression in 10-month-old SAMP8 versus 5-month-old SAMP8. One most significantly dysregulated circRNA, mmu_circRNA_017963, was select for Gene Oncology (GO) and pathway analysis. The results showed that mmu_circRNA_017963 was strongly related with autophagosome assembly, exocytosis, apoptotic process, transport and RNA splicing and highly associated with synaptic vesicle cycle, spliceosome, glycosaminoglycan and SNARE interactions in vesicular transport pathways. Collectively, this study was the first to describe circRNAs expression in different ages of SAMP8 and will contribute to the understanding of the regulatory roles of circRNAs in AD pathogenesis and provide a valuable resource for the diagnosis and therapy of AD.