Transmembrane domain length is responsible for the ability of a plant reticulon to shape endoplasmic reticulum tubules in vivo.
Transmembrane domain length is responsible for the ability of a plant reticulon to shape endoplasmic reticulum tubules in vivo.
复制标题
跨膜结构域长度决定植物网网在体内塑造内质网小管的能力。
DOI：
10.1111/j.1365-313x.2010.04337.x
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Tolley N
中科院分区：
文献类型：
--
作者：
Tolley N
网状蛋白是一种完整的内质网（ER）膜蛋白，具有将内质网塑造成小管的能力。据推测，它们异常长的保守疏水区域导致网状结构呈现楔形拓扑结构，从而诱导膜曲率。在这里，我们提供了这一假设的证据。当过表达时，拟南芥（RTNLB13）定位于皮质内质网小管，并诱导其收缩。在拟南芥突变体（pah1 pah2）中，RTNLB13的异位表达足以诱导内质网管化，其内质网膜主要以片状形式存在。通过连续缩短RTNLB13的四个跨膜结构域（TMDs），我们发现跨膜区域的长度与RTNLB13诱导膜管化和在内质膜内形成低迁移率复合物的能力直接相关。我们还表明，全长tmd对于RTNLB13在内质网膜上的驻留能力是必要的。
Reticulons are integral endoplasmic reticulum (ER) membrane proteins that have the ability to shape the ER into tubules. It has been hypothesized that their unusually long conserved hydrophobic regions cause reticulons to assume a wedge‐like topology that induces membrane curvature. Here we provide proof of this hypothesis. When over‐expressed, anArabidopsis thalianareticulon (RTNLB13) localized to, and induced constrictions in, cortical ER tubules. Ectopic expression of RTNLB13 was sufficient to induce ER tubulation in an Arabidopsis mutant (pah1 pah2) whose ER membrane is mostly present in a sheet‐like form. By sequential shortening of the four transmembrane domains (TMDs) of RTNLB13, we show that the length of the transmembrane regions is directly correlated with the ability of RTNLB13 to induce membrane tubulation and to form low‐mobility complexes within the ER membrane. We also show that full‐length TMDs are necessary for the ability of RTNLB13 to reside in the ER membrane.