Cytoplasmic male sterility in the olive (Olea europaea L.)
Cytoplasmic male sterility in the olive (Olea europaea L.)
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DOI：
10.1007/s001220051383
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发表时间：
2000-05-01
影响因子：
5.4
通讯作者：
Bervillé, A
中科院分区：
文献类型：
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作者：
Besnard, G;Khadari, B;Bervillé, A
橄榄树通常是雌雄同体，但自交不亲和。在西地中海，一些品种完全雄性不育。三种不同的雄性不育表型已被确认。为了推断雄性不育的遗传基础，我们研究了野生（沙枣）和栽培地中海橄榄的遗传和细胞质多样性。在OlivierexArbequinainn杂交中，雄性不育性状为母系遗传，影响所有后代。我们还检查了叶绿体和线粒体DNA都是母系遗传的。对叶绿体和线粒体DNA的RFLP研究揭示了几种细胞型：两个品种和沙枣（野生或野生地中海橄榄）的叶绿体型和四个有丝分裂型。此外，CCK氯型和MCK有丝分裂型在不同地区的品种和沙枣之间的总连锁失衡支持的事实是，没有观察到父本泄漏的细胞器。Oliviere等6个品种和3个沙枣的雄性不育（ms2）与CCK氯型和MCK有丝分裂型密切相关。这些事实表明，Oliviere携带细胞质雄性不育。携带该细胞型的雄性可育和雄性不育的油菜均存在恢复等位基因。这种CMS可能是由于橄榄类群之间的远缘杂交。Lucques（ms 1）和Tanche（ms 3）显示的另外两种雄性不育表型与ME 1有丝分裂型相关，但我们尚未证明CMS。
The olive tree is usually hermaphrodite but self-incompatible. In the Western Mediterranean some cultivars are totally male-sterile. Three different male-sterile phenotypes have been recognised. To infer the genetic basis of male sterility we studied its inheritance and cytoplasmic diversity in wild (oleaster) and cultivated Mediterranean olive. In the cross OlivierexArbequina inn, the male-sterile trait was maternally inherited and affected all progenies. We also checked that both chloroplast and mitochondrial DNAs are maternally inherited. RFLP studies on chloroplast and mitochondrial DNAs revealed several cytotypes: two chlorotypes and four mitotypes in cultivars and oleaster (wild or feral Mediterranean olive). Furthermore, a total linkage desequilibrium between the CCK chlorotype and the MCK mitotype in cultivars and oleaster from different regions supports the fact that paternal leakage of organelles was not observed. The male sterility (ms 2) displayed by Oliviere plus six other cultivars and three oleaster was strictly associated with the CCK chlorotype and the MCK mitotype. These facts suggest that Oliviere carries cytoplasmic male sterility. Male-fertile and male-sterile oleasters carrying this cytotype showed the presence of restorer alleles. This CMS might be due to a distant cross between olive taxa. The two other male-sterile phenotypes displayed by Lucques (ms 1) and Tanche (ms 3) were associated with the ME1 mitotype but we have not demonstrated CMS.