Enhancement of rotavirus immunogenicity by microencapsulation.
Enhancement of rotavirus immunogenicity by microencapsulation.
复制标题
通过微胶囊化增强轮状病毒的免疫原性。
DOI：
10.1006/viro.1994.1463
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发表时间：
1994
期刊：
影响因子：
3.7
通讯作者：
Speaker,TJ
中科院分区：
文献类型：
--
作者：
Offit,PA;Khoury,CA;Moser,CA;Clark,HF;Kim,JE;Speaker,TJ
研究了轮状病毒微囊化是否能增强小鼠的病毒特异性免疫。几种水溶性阴离子聚合物和胺的组合测试了它们在模拟胃酸存在下形成微胶囊的能力。将海藻酸钠与盐酸精胺或硫酸软骨素钠与盐酸精胺联合使用，我们发现微胶囊(1)捕获了传染性轮状病毒，(2)口服接种后进入了持续存在的肠相关淋巴组织（GALT），(3)将轮状病毒抗原递送到GALT，其水平高于口服接种游离病毒后检测到的水平。(4)口服或肠外免疫后增强了病毒特异性体液免疫应答。
It was determined whether microencapsulation of rotavirus enhanced virus-specific Immunity in mice. Combinations of several water-soluble anionic polymers and amines were tested for their capacity to form microcapsules which were stable in the presence of simulated gastric acid. Using the combinations of sodium alginate and spermine hydrochloride or sodium chondroitin sulfate and spermine hydrochloride we found that microcapsules (1) captured infectious rotavirus, (2) penetrated Into the persisted in gut-associated lymphold tissue (GALT) after oral inoculation, (3) delivered rotavirus antigen to GALT at levels greater then those detected after oral inoculation with free virus, and (4) enhanced the virus-specific humoral immune response after oral or parenteral immunization.