In vitro assembly of the mouse U14 snoRNP core complex and identification of a 65-kDa box C/D-binding protein.
In vitro assembly of the mouse U14 snoRNP core complex and identification of a 65-kDa box C/D-binding protein.
复制标题
小鼠 U14 snoRNP 核心复合物的体外组装和 65 kDa 盒 C/D 结合蛋白的鉴定。
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
E. Maxwell
中科院分区：
文献类型：
--
作者：
N. Watkins;D. Newman;J. F. Kuhn;E. Maxwell
The eukaryotic nucleolus contains a diverse population of small nucleolar RNAs (snoRNAs) that have been categorized into two major families based on evolutionarily conserved sequence elements. U14 snoRNA is a member of the larger, box C/D snoRNA family and possesses nucleotide box C and D consensus sequences. In previous studies, we have defined a U14 box C/D core motif that is essential for intronic U14 snoRNA processing. These studies also revealed that nuclear proteins that recognize boxes C/D are required. We have now established an in vitro U14 snoRNP assembly system to characterize protein binding. Electrophoretic mobility-shift analysis demonstrated that all the sequences and structures of the box C/D core motif required for U14 processing are also necessary for protein binding and snoRNP assembly. These required elements include a base paired 5',3' terminal stem and the phylogenetically conserved nucleotides of boxes C and D. The ability of other box C/D snoRNAs to compete for protein binding demonstrated that the box C/D core motif-binding proteins are common to this family of snoRNAs. UV crosslinking of nuclear proteins bound to the U14 core motif identified a 65-kDa mouse snoRNP protein that requires boxes C and D for binding. Two additional core motif proteins of 55 and 50 kDa were also identified by biochemical fractionation of the in vitro-assembled U14 snoRNP complex. Thus, the U14 snoRNP core complex is a multiprotein particle whose assembly requires nucleotide boxes C and D.
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DOI：
10.1073/pnas.93.25.14480
发表时间：
1996-12-10
影响因子：
11.1
作者：
Tycowski, KT;Smith, CM;Steitz, JA
通讯作者：
Steitz, JA
影响因子：
10.5
作者：
TYCOWSKI, KT;SHU, MD;STEITZ, JA
通讯作者：
STEITZ, JA
影响因子：
11.4
作者：
BASERGA, SJ;YANG, XDW;STEITZ, JA
通讯作者：
STEITZ, JA
影响因子：
10.5
作者：
S. Baserga;M. Gilmore-Hebert;X. W. Yang
通讯作者：
S. Baserga;M. Gilmore-Hebert;X. W. Yang
DOI：
10.1002/j.1460-2075.1995.tb00167.x
发表时间：
1995-10
期刊：
The EMBO Journal
影响因子：
--
作者：
M. Terns;C. Grimm;E. Lund;J. Dahlberg
通讯作者：
M. Terns;C. Grimm;E. Lund;J. Dahlberg