The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis.
The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis.
复制标题
短期高脂肪喂养对运动能力的影响：通过 RNA 测序分析多组织转录组变化。
DOI：
10.1186/s12944-017-0424-7
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发表时间：
2017-02-02
影响因子：
4.5
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Xiao Y;Wang W;Chen L;Chen J;Jiang P;Fu X;Nie X;Kwan H;Liu Y;Zhao X
短期高脂肪饮食对生理的影响是难以捉摸的，脂肪过度消耗后的分子变化在很大程度上仍然未知。在这项研究中，我们旨在评估高脂肪饮食（HFD）喂养3天的小鼠的运动能力，并探讨高脂肪喂养早期反应的分子机制。通过体重负荷游泳试验评估小鼠的运动能力，小鼠喂食对照饮食（10千卡脂肪%）或高热量饮食（60千卡脂肪%）3天。采用RNA测序技术分析10个重要组织（脑、心、肝、脾、肺、肾、胃、十二指肠、骨骼肌和血液）的整体基因表达。在体重显著增加之前，仅3天的HFD可导致71%的运动表现下降（P <0.01）。主成分分析显示，10种组织中存在差异的基因表达模式。其中，脑、脾和肺比其他组织有更明显的转录变化。对脑、脾和肺中差异表达基因的生物学过程分析表明，外周和中枢免疫反应的失调与HFD暴露的早期阶段有关。脑和肺中神经传递相关基因和循环系统过程相关基因分别显著下调。我们的发现为高脂肪喂养的有害作用提供了新的见解，特别是揭示了肺可能是短期高脂肪喂养攻击的一个新的重要目标。本文的在线版本（doi:10.1186/s12944-017-0424-7）包含补充材料，可供授权用户使用。
The effects of short-term high fat diets on physiology are elusive and the molecular changes following fat overconsumption remain largely unknown. In this study, we aimed to evaluate exercise capacity in mice fed with a high fat diet (HFD) for 3 days and investigate the molecular mechanisms in the early response to high-fat feeding. Exercise capacity was assessed by weight-loaded swimming test in mice fed a control diet (10 kcal% fat) or a HFD (60 kcal% fat) for 3 days. Global gene expression of ten important tissues (brain, heart, liver, spleen, lung, kidney, stomach, duodenum, skeletal muscle and blood) was analyzed using RNA Sequencing. A HFD for just 3 days can induce 71% decrease of exercise performance prior to substantial weight gain (P <0.01). Principle component analysis revealed that differential gene expression patterns existed in the ten tissues. Out of which, the brain, spleen and lung were demonstrated to have more pronounced transcriptional changes than other tissues. Biological process analysis for differentially expressed genes in the brain, spleen and lung showed that dysregulation of peripheral and central immune response had been implicated in the early stage of HFD exposure. Neurotransmission related genes and circulatory system process related genes were significantly down-regulated in the brain and lung, respectively. Our findings provide new insights for the deleterious effects of high-fat feeding, especially revealing that the lung maybe as a new important target attacked by short-term high-fat feeding. The online version of this article (doi:10.1186/s12944-017-0424-7) contains supplementary material, which is available to authorized users.