Correlation analysis between grain color and cyanidin-3-glucoside content of rice grain in segregate population.
Correlation analysis between grain color and cyanidin-3-glucoside content of rice grain in segregate population.
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DOI：
10.9787/pbb.2015.3.2.160
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Koh, H. J.
中科院分区：
文献类型：
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作者：
Ham TaeHo, Ham TaeHo;Kwon SoonWook, Kwon SoonWook;Koh, H. J.
研究了黑紫米花色素3-葡萄糖苷（C3 G）含量的遗传变异。以黑紫稻与普通白色稻杂交建立F2群体。所用的微黑稻品种为吉林黑蜜、黑稻38、LK 1A-2-12-1-1、黑津株和2号，所用的普通白色稻品种为花中超级巨胚和黑宝。紫色果皮颜色是由一组显性等位基因Pb（Prp-b）和Pp（Prp-a）控制的。在本研究中，黑紫：棕色：白色果皮在F2群体中的分离比例为9：3：4，说明紫色果皮性状受两对显性互补基因控制。C3 G在F2代的分布表现为连续变异，各组合均呈低水平分布。而吉林黑蜜/黑稻38、吉林黑蜜/LK 1A-2-12-1-1等F_2代C_3G含量表现为超亲分离。吉林黑蜜与黑稻38的F2群体C3 G含量在84%水平上连锁，与LK 1A-2-12-1-1的F2群体C3 G含量在41.3%水平上连锁。色差计和C3 G含量的HPLC分析结果表明，有色稻种质的种皮颜色与分离群体的种皮颜色之间存在极显著的相关性。C3 G含量与L*（亮度）和B*（黄度）呈显著正相关。红度a* 在各杂交组合间存在差异，但C3 G含量与L* 和B* 呈显著正相关。
This study examined the genetic variation of cyanidin 3-glucoside (C3 G) contents in blackish-purple rice. F2 populations were established from crosses between blackish purple rice and normal white rice. The blackish rice cultivars used were Jilinheimi, Heidao38, LK1A-2-12-1-1, Heugjinju, and No2, and the common white rice cultivars used were Hwachung super giant embryo and Heugbal. The purple pericarp color is known to be controlled by a set of dominant alleles, Pb(Prp-b) and Pp(Prp-a). In this study, the segregation of the black purple: brown: white pericarp in the F2 population was 9:3:4, demonstrating that the purple pericarp color was controlled by two dominant complementary genes. The F2 distribution of C3 G showed continuous variation, with a tendency toward a lower level in all the crosses. However, some F2 crosses, such as Jilinheimi/Heidao38 and Jilinheimi/LK1A-2-12-1-1, showed transgressive segregation of the C3G content. C3G content of Jilinheimi linked on 84% level in C3G content of F2 population between Jilinheimi/Heidao38, and 41.3% level in Jilinheimi/LK1A-2-12-1-1. The results of the color difference meter and C3G content analysed by HPLC revealed highly significant correlations between the seed coat color of the colored rice germplasm and that of the segregated populations. There was a significant positive correlation between the C3G content with L* (lightness) and b* (yellowness). The a* (redness) was different in each cross, but there was a significant positive correlation between the C3G content with L* and b*.