Karyotype relationships of six bat species (Chiroptera, Vespertilionidae) from China revealed by chromosome painting and G-banding comparison
Karyotype relationships of six bat species (Chiroptera, Vespertilionidae) from China revealed by chromosome painting and G-banding comparison
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DOI：
10.1159/000095235
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发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Nie, W.
中科院分区：
文献类型：
--
作者：
Ao, L.;Gu, X.;Nie, W.
蝶翅科是翼翅目中最大的科，分布在温带和热带地区。为了进一步明确狐蝠科低分类水平上的核型关系，本文构建了6只中国狐蝠与Myotis Myotis （MMY, 2n = 44）的全基因组比较图谱。利用一套源自于Myotis Myotis流分选染色体的染色探针进行跨种染色体染色，对牛腹肌（MAL, 2n = 44）、粉状小叶蝉（HPU, 2n = 44）、绒皮Nyctalus （NVE, 2n = 36）、robustula Tylonycteris （TRO, 2n = 32）、Tylonycteris sp. （TSP, 2n = 30）和富利小叶蝉（MFU, 2n = 46）进行了染色。每个Myotis Myotis常染色体探针在这6只蝙蝠的基因组中检测到一个同源染色体片段，除了MMY染色体3/4 paint杂交到M. fuliginosus基因组的两条染色体上。结果表明，Robertsonian易位是虎蛛科核型进化的主要模式，异色物质的加入在虎蛛属和夜蛛属的核型进化中也起着重要作用。MMY9 + 23和18 + 19可能是Tylonycteris属的突触性特征。将我们的图谱与已发表的图谱相结合，使我们能够推断出人类与这六种蝙蝠之间的染色体同源性，并为蝙蝠科的核型进化提供了新的见解。版权所有(c) 2006 S. Karger AG，巴塞尔。
The Vespertilionidae is the largest family in the order Chiroptera and has a worldwide distribution in the temperate and tropical regions. In order to further clarify the karyotype relationships at the lower taxonomic level in Vespertilionidae, genome-wide comparative maps have been constructed between Myotis myotis (MMY, 2n = 44) and six vesper bats from China: Myotis altarium (MAL, 2n = 44), Hypsugo pulveratus (HPU, 2n = 44), Nyctalus velutinus (NVE, 2n = 36), Tylonycteris robustula (TRO, 2n = 32), Tylonycteris sp. (TSP, 2n = 30) and Miniopterus fuliginosus (MFU, 2n = 46) by cross-species chromosome painting with a set of painting probes derived from flow-sorted chromosomes of Myotis myotis. Each Myotis myotis autosomal probe detected a single homologous chromosomal segment in the genomes of these six vesper bats except for MMY chromosome 3/4 paint which hybridized onto two chromosomes in the genome of M. fuliginosus. Our results show that Robertsonian translocation is the main mode of karyotype evolution in Vespertilionidae and that the addition of heterochromatic material also plays an important role in the karyotypic evolution of the genera Tylonycteris and Nyctalus. Two conserved syntenic associations (MMY9 + 23 and 18 + 19) could be the synapomorphic features for the genus Tylonycteris. The integration of our maps with the published maps has enabled us to deduce chromosomal homologies between human and these six vesper bats and provided new insight into the karyotype evolution of the family Vespertilionidae. Copyright (c) 2006 S. Karger AG, Basel.