Elucidating the role of SlXTH5 in tomato fruit softening
Elucidating the role of SlXTH5 in tomato fruit softening
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DOI：
10.1016/j.hpj.2022.12.005
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发表时间：
2022-12
影响因子：
5.7
通讯作者：
Duoduo Wang;Qianhui Lu;Xiaomin Wang;Hui Ling;Ning Huang
中科院分区：
文献类型：
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作者：
Duoduo Wang;Qianhui Lu;Xiaomin Wang;Hui Ling;Ning Huang
番茄果实软化与细胞壁的解体密切相关，细胞壁的解体是通过一系列与细胞壁结构相关的酶和其他蛋白质如Expansins的作用而实现的。木葡聚糖酶/水解酶(XTH)(EC 2.4.1.207和/或EC 3.2.1.151)是参与木葡聚糖代谢的关键基因，其中SLXTH5在番茄成熟过程中的表达水平最高。本研究以基于CRISPR的SLXTH5基因敲除突变体为材料，研究了SLXTH5在番茄软化过程中的作用。与杂合野生型(WT)对照相比，转基因番茄中SLXTH5功能缺失导致果皮略硬，但其颜色指数显著降低。在CRISPR突变体中检测到糊糊粘度增加，这表明SLXTH5的活性负责维持细胞壁结构的完整性。用单抗探针LM25进行了免疫细胞化学研究，检测了木葡聚糖在CRISPR突变果实果皮细胞中的定位和分布。结果表明，CRISPR突变体果实果皮中的木葡聚糖含量高于对照果实。本研究揭示了SLXTH5与木聚糖代谢之间的联系，揭示了SLXTH5调控果实软化的可能性。。
Fruit softening in tomato (Solanum lycopersicum) is closely associated with cell wall disassembly, which is brought about through the action of a range of cell wall structure-related enzymes and other proteins such as expansins. Xyloglucan endotransglucosylase/hydrolase (XTH) (EC 2.4.1.207 and/or EC 3.2.1.151) has been proposed to be key player involved in xyloglucan metabolism.SlXTH5showed the highest expression level among allSlXTHsduring tomato ripening. In this study, the role ofSlXTH5involved in tomato softening was investigated in CRISPR-based knockout mutants ofSlXTH5. Loss-of-function ofSlXTH5in transgenic tomato lines resulted in slightly firmer fruit pericarp, but significantly decreased their color index compared with azygous wild type (WT) control fruits. Increased paste viscosity was detected in CRISPR mutants, indicating that the activity of SlXTH5 is responsible for maintaining cell wall structural integrity. Immunocytochemistry studies were performed using the monoclonal antibody probe LM25 to examine the localization and distribution of xyloglucan in the pericarp cells of the CRISPR mutant fruits. The data indicated more xyloglucan was retained in the pericarp of CRISPR mutant fruit than in WT control fruit. This study revealed the link betweenSlXTH5and xyloglucan metabolism and indicated the potential of manipulatingSlXTH5to regulate fruit softening. .