Rho kinase regulates neurite outgrowth of hippocampal neurons via calcium dependent cytoskeleton regulation.
Rho kinase regulates neurite outgrowth of hippocampal neurons via calcium dependent cytoskeleton regulation.
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发表时间：
2017
影响因子：
2.2
通讯作者：
Zhisheng Ji;Zhenbin Cai;Jifeng Zhang;Nannuan Liu;Jing Chen;Minghui Tan;Hongsheng Lin;Guoqing Guo
中科院分区：
文献类型：
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作者：
Zhisheng Ji;Zhenbin Cai;Jifeng Zhang;Nannuan Liu;Jing Chen;Minghui Tan;Hongsheng Lin;Guoqing Guo
目的探讨钙离子是否参与Rho激酶调控神经突起生长的下游信号转导。方法体外培养原代海马神经元，分别用Rho激酶激动剂（LPA）和拮抗剂Y-27632处理。观察细胞骨架和突起生长情况。加入钙拮抗剂BAPTA/AM降低细胞内钙后，观察细胞骨架分布和突起生长。结果Rho激酶的激活或抑制均能显著改变海马神经元突起的数量和长度。Rho激酶通过调节细胞骨架来调节突起的生长，LPA能显著增加细胞内钙离子浓度。BAPTA/AM处理后神经元突起长度和分支数明显减少。BAPTA/AM能降低神经细胞内钙离子浓度，减少神经细胞骨架。BAPTA/AM和LPA均能阻止神经突起的回缩，但Y-27632和LPA处理后神经突起的长度和分支数无明显变化。结论Ca ~（2+）可能通过影响细胞骨架的排列来调控突起的生长，Ca ~（2+）参与了Rho激酶调控海马神经元突起生长的下游信号转导。
OBJECTIVE To investigate whether calcium is involved in downstream signal transduction in neurite outgrowth regulated by Rho kinase. METHODS In vitro primary hippocampal neurons were cultured and treated with Rho kinase agonist (LPA) or antagonist (Y-27632). Then, the cytoskeleton and neurite outgrowth were observed. After addition of calcium antagonist BAPTA/AM to reduce intracellular calcium, the cytoskeleton distribution and neurite outgrowth were observed. RESULTS The activation or inhibition of Rho kinase could significantly alter the number and length of neurites of hippocampal neurons. Rho kinase regulated the cytoskeleton to regulate the neurite outgrowth, and LPA could significantly increase intracellular calcium. After BAPTA/AM treatment, the length and branch number of neurites of neurons reduced markedly. BAPTA/AM was able to reduce intracellular calcium and decrease neuronal cytoskeleton. Treatment with both BAPTA/AM and LPA could stop the retraction of neurites, but the length and branch number of neurites remained unchanged after treatment with Y-27632 and LPA. CONCLUSION Calcium may affect the cytoskeleton arrangement to regulate neurite outgrowth, and calcium is involved in the downstream signal transduction of Rho kinase regulated neurite outgrowth of hippocampal neurons.