EBV INFECTION OF MITOGEN-STIMULATED HUMAN LYMPHOCYTES-B
EBV INFECTION OF MITOGEN-STIMULATED HUMAN LYMPHOCYTES-B
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DOI：
10.1002/ijc.2910270209
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发表时间：
1981-01-01
影响因子：
6.4
通讯作者：
KLEIN, E
中科院分区：
文献类型：
--
作者：
EINHORN, L;KLEIN, E
用丝裂原蛋白A（金黄色葡萄球菌）处理来自人血液的B淋巴细胞，所述丝裂原蛋白A诱导B淋巴细胞中的DNA合成以及分化。随后将细胞暴露于Epstain-Barr病毒（EBV）。通过联合使用免疫荧光（用于EBNA [EBV核抗原]）和放射自显影（用于DNA合成），同时检测EBV感染和促分裂原刺激的细胞。在加入有丝分裂原后的初始阶段（1-2天），受刺激的细胞与静息细胞一样易受感染。此后，他们的情绪逐渐低落。尽管DNA合成的起始似乎并不限制对病毒感染的敏感性，但B细胞的分化可能会限制对病毒感染的敏感性。EBNA染色的强度和模式在预刺激的细胞不同于对照组。
B lymphocytes from human blood were treated with the mitogen Protein A (Staphylococcus, aureus), which induces DNA synthesis in, as well as differentiation of, the B lympohocytes. The cells were subsequently exposed to Epstain-Barr virus (EBV). EBV-infected and mitogen-stimulated cells were detected simultaneously by using a combination of immunofluorescence (for EBNA [EBV nuclear antigen]) and autoradiography (for DNA-synthesis). In the initial phase (1-2 days) after addition of the mitogen, stimulated cells were as susceptible to infection as resting cells. Thereafter, their susceptibility decreased. Although initiation of DNA-synthesis does not seem to limit sensitivity to the viral infection, differentiation of the B cells might do so. The intensity and pattern of EBNA-staining in prestimulated cells differed from that of the controls.