PROPERTIES OF LIQUID BILAYER MEMBRANES SEPARATING 2 AQUEOUS PHASES - TEMPERATURE DEPENDENCE OF WATER PERMEABILITY
PROPERTIES OF LIQUID BILAYER MEMBRANES SEPARATING 2 AQUEOUS PHASES - TEMPERATURE DEPENDENCE OF WATER PERMEABILITY
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DOI：
10.1016/0022-2836(69)90287-3
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发表时间：
1969-01-01
影响因子：
5.6
通讯作者：
THOMPSON, TE
中科院分区：
文献类型：
--
作者：
PRICE, HD;THOMPSON, TE
通过测量NaCl梯度产生的净体积通量，确定了脂质双层膜水渗透性在7 ~ 49°C范围内的温度依赖性。膜由十烷中的卵磷脂和胆固醇溶液生成，0.5%（w/v）卵磷脂和0.25%胆固醇（溶液A）或1%卵磷脂和1%胆固醇（溶液B）。25℃时，溶液A和溶液B的渗透系数分别为31.7±4.3 μ/sec和24.4±3.7 μ/sec。In （P os）与1 T呈线性关系，实验活化能ea的产率分别为12.7±0.3和13.1±0.7 kcal./mol。这些结果与文献中关于水在碳氢化合物中的溶解度和扩散的数据一致，证明了水渗透过程的简单溶解度-扩散机制。细胞膜和脂质双分子层的pos和ea值之间的相似性表明，生物系统中可能存在类似的机制。
The temperature dependence of the water permeability of lipid bilayer membranes has been determined over the interval 7 to 49° C, by measuring the net volume flux produced by a NaCl gradient. The membranes were generated from solutions of lecithin and cholesterol in decane, either 0.5%(w/v) lecithin and 0.25% cholesterol (solution A) or 1% lecithin and 1% cholesterol (solution B). At 25° C, P os, the osmotic permeability coefficient, is 31.7±4.3 μ/sec and 24.4±3.7 μ/sec for membranes formed from solutions A and B, respectively. Plots of In (P os) versus 1 T are linear and yield values of 12.7±0.3 and 13.1±0.7 kcal./mole for the experimental activation energies E a. These results, together with literature data for the solubility and diffusion of water in hydrocarbons, are consistent with a simple solubility-diffusion mechanism for the water permeation process. The similarity between the values of both P os and E a for cellular membranes and the lipid bilayer suggests that a similar mechanism may operate in the biological systems.